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REVIEW ARTICLE
Year : 2017  |  Volume : 3  |  Issue : 3  |  Page : 18-27

Monoclonal antibody usage strategies for natural products in traditional chinese medicine


Department of Pharmacognosy, Faculty of Pharmaceutical Science, Nagasaki International University, Sasebo, Nagasaki 859-3298, Japan

Correspondence Address:
Yukihiro Shoyama
Department of Pharmacognosy, Faculty of Pharmaceutical Science, Nagasaki International University, Sasebo, Nagasaki 859-3298
Japan
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/wjtcm.wjtcm_5_17

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Monoclonal antibodies (MAbs) against natural products with low-molecular weights have become an important tool when combined with other analytical systems. Eastern blotting involves a typical staining system wherein, for example, glycosides can be blotted to a membrane and cross-linked and stained using MAbs. An immunoaffinity column combined with a monoclonal antibody allows a one-step purification of hapten compounds or preparation of a knockout extract that removes only the target hapten molecules from a crude extract. Here, we discuss the application of these extracts. Single-chain variable fragment (scFv) proteins have led to novel assay systems such as fluobodies or antibodies coupled with green fluorescent protein for natural products. A typical novel application of a scFv gene would be in the context of plant breeding; this is designated “missile-type” molecular breeding, intended to increase hapten molecule concentrations in transgenic plants.


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