• Users Online: 87
  • Print this page
  • Email this page
ORIGINAL ARTICLE
Year : 2017  |  Volume : 3  |  Issue : 4  |  Page : 21-25

The protective effect of Gan Shen Fu Fang on liver endothelial cells in common bile duct-ligated rats


Department of Pathology, School of Preclinical Medicine, Beijing University of Chinese Medicine, Beijing 100029, China

Correspondence Address:
Prof. Qing-Hong Du
School of Preclinical Medicine, Beijing University of Chinese Medicine, 11 Bei San Huan Dong Lu, Beijing 100029
China
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/wjtcm.wjtcm_16_17

Rights and Permissions

Objective: To elucidate the protective effect of Gan Shen Fu Fang (GSFF) on liver endothelial cells in common bile duct-ligated (CBDL) rats. Materials and Methods: Cirrhosis was induced by common bile duct ligation. The rats were divided into three groups: sham group, CBDL group, and GSFF group. After 2 weeks of ligation, rats in the GSFF group were administered GSFF. After 4 weeks, the hydroxyproline (Hyp) content of liver tissues was spectrophotometrically determined. The histological changes were evaluated by H and E and Masson staining. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) were used to observe the ultrastructural changes in the liver, especially in the liver sinusoidal endothelial cells (LSECs). Results: Hyp synthesis was significantly inhibited by GSFF, which agreed with the results from H and E and Masson staining for liver fibrosis. The TEM observations of CBDL rats revealed reduced hepatocyte microvilli and deposited fibrous tissue underneath LSECs. SEM confirmed the TEM findings and showed that the fenestrae of LSECs decreased and even disappeared in CBDL rats. The morphological results indicated hepatic sinusoid capillarization. GSFF promoted the restoration of fenestrae and reversed hepatic sinusoid capillarization. Conclusion: GSFF can inhibit Hyp synthesis, restore the fenestrae of LSECs, and reverse hepatic sinusoid capillarization in CBDL rats. These results provide a basis for future detailed investigations of the mechanism of action of GSFF in LSECs.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed394    
    Printed49    
    Emailed0    
    PDF Downloaded76    
    Comments [Add]    

Recommend this journal