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ORIGINAL ARTICLE
Year : 2019  |  Volume : 5  |  Issue : 4  |  Page : 236-242

Purification and identification of a novel protein isolated from Panax quinquefolium and evaluation of its In vitro antioxidant properties


1 Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun, China
2 College of Pharmacy, Changchun University of Chinese Medicine, Changchun, China

Correspondence Address:
Prof. Bin Qi
College of Pharmacy, Changchun University of Chinese Medicine, Changchun, Jilin, 130117
China
Prof. Li Liu
College of Pharmacy, Changchun University of Chinese Medicine, Changchun, Jilin,130117
China
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/wjtcm.wjtcm_20_19

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Objective: A novel protein was first purified from Panax quinquefolius L. (AGNP), and in vitro antioxidant activities of AGNP were first studied in this work. Methods: AGNP was purified by Ion-exchange chromatography and Gel-filtration chromatography. The chemical characterizations of AGNP were tested by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE), high-pressure gel-filtration chromatography, MALDI-TOF-MS and HPLC. In vitro antioxidant effects were tested in simple antioxidant assay including 2,2-diphenylpicrylhydrazyl radical scavenging, superoxide radical (O2) scavenging, hydroxyl radical (OH) scavenging, and ferric-reducing ability. Results: The results showed which the content of AGNP measured by Bradford method was 2.42 mg/mL and the subunit molecular weight of AGNP measured by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) was 15 kD. The AGNP molecular weight was15, 114 Da both of SDS-PAGE and mass spectrum purity. The result of high-pressure gel-filtration chromatography demonstrated that the molecular weight of AGNP was 31,086 Da, which implied that AGNP was a homodimer. The in vitro Antioxidant results indicated that AGNP had obvious effects to remove the free radicals in vitro. Conclusion: In conclusion, AGNP had more powerful antioxidant capacity and it can be used as an effective natural antioxidant to alleviate oxidative stress.


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