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ORIGINAL ARTICLE
Year : 2020  |  Volume : 6  |  Issue : 3  |  Page : 324-330

Analysis of phytochemical constituents of zuogui wan in rat serum and its effects on early embryonic development of mice


1 Institute of Chinese and Food Engineering, Shanxi University of Chinese Medicine, Jinzhong, China
2 College of Basic Medical Sciences, Shanxi University of Chinese Medicine, Jinzhong, China
3 School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing, China
4 School of Chemical Engineering and Advanced Materials, The University of Adelaide, Adelaide, Australia

Correspondence Address:
Prof. Ying-Li Wang
Shanxi University of Chinese Medicine, Jinzhong 030619
China
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/wjtcm.wjtcm_34_20

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Objective: Zuogui Wan (ZGW) has been used as a typical prescription for tonifying kidney essence in traditional Chinese medicine. The objective of this study is to elucidate the phytochemical constituents of ZGW-treated rat serum (ZGWRS) using ultra-performance liquid chromatography-electrospray ionization/quadrupole-time-of-flight high-definition mass spectrometry (UPLC-ESI-Q-TOF-MS). Methods: ZGW was administered to rats, and the phytochemical constituents in rat serum were determined using UPLC-ESI-Q-TOF-MS. MetaboLynx analysis in negative ion mode was adopted to characterize the chemical constituents of ZGWRS. Orthogonal partial least squares discriminant analysis was applied for the discovery of constituents of ZGW that entered the serum of rats. The fertilized eggs collected from the same experiment were randomly divided into four groups, including the normal, 40 mmol/L glucose, 40 mmol/L glucose 5% control rat serum, and 40 mmol/L glucose 5% ZGWRS groups. They were cultivated at 37°C and 5% CO2in a saturated humidity CO2incubator. The blastocyst rate and two-cell rate were used to evaluate the effects of ZGWRS on embryonic development. Results: Thirteen constituents were identified in the ZGWRS, among which sweroside, loganin, morroniside, loganic acid, and 8-epiloganic acid were from Fructus Corni (Shan Zhu Yu). 5-Hydroxymethyl-2-furfural-glucuronide, 2-H-5-hydroxymethyl-2-furfural-glucuronide, 3-hydroxy-2,6,6-trimethyl-1-cyclohexene-1-carboxylic acid, and β-D-ribofuranuronic acid methyl ester triacetate were from Radix Rehmanniae Preparata (Shu Di Huang). Coumaric acid was from Fructus Lycii (Gou Qi Zi). Kaempferol-3-beta-O-glucuronide and cuscutamine were from Semen Cuscutae (Tu Si Zi). The embryonic development was significantly inhibited using 40 mmol/L glucose. Compared with the normal group, the blastocyst rate of the glucose group was decreased. The blastocyst rate of the 40 mmol/L glucose 5% ZGWRS group was significantly higher than that of the glucose group, indicating that ZGWRS negates the effect of glucose on mouse embryonic development. Conclusion: The results verified that a rapid and robust UPLC-ESI-Q-TOF-MS-based platform had been successful for identifying multiple constituents of ZGW. ZGWRS is rich in active constituents of iridoid glycosides. The results of this study also showed that ZGWRS could negate the effect of glucose on mouse embryonic development.


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