|Year : 2020 | Volume
| Issue : 4 | Page : 393-407
Medicinal plant of Bletilla striata: A review of its chemical constituents, pharmacological activities, and quality control
Sai Jiang1, Meng-Yun Wang1, Han-Wen Yuan1, Qian Xie1, Yang Liu1, Bo-Shu Li1, Yu-Qing Jian1, Chang-Xiao Liu2, Hua-Yong Lou3, Atta-Ur-Rahman4, Wei-Dong Pan3, Wei Wang1
1 TCM and Ethnomedicine Innovation and Development International Laboratory, School of Pharmacy, Innovative Materia Medica Research Institute, Hunan University of Chinese Medicine, Changsha, China
2 Research Center for New Drug Evaluation, Research Center for Modern Chinese Medicines, Tianjin Institute of Pharmaceutical Research, Tianjin, China
3 State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang, China
4 H E J Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan
|Date of Submission||21-May-2020|
|Date of Acceptance||22-Jun-2020|
|Date of Web Publication||15-Oct-2020|
Prof. Wei Wang
TCM and Ethnomedicine Innovation and Development International Laboratory, School of Pharmacy, Innovative Materia Medica Research Institute, Hunan University of Chinese Medicine, Changsha
Source of Support: None, Conflict of Interest: None
Bletilla striata belongs to the family Orchidaceae, and it is mainly distributed in East Asia. The tubers of B. striata have been utilized in Traditional Chinese Medicine for various ailments, such as hematemesis, tuberculosis, malignant ulcers, hemorrhoids, traumatic bleeding, and chapped skin. Phytochemical investigation on B. striata has resulted in the identification of 192 monomeric compounds, including bibenzyls, phenanthrene derivatives, triterpenoids and its saponins, steroids and its saponins, malic acid derivatives, and anthocyanins. Moreover, B. striata polysaccharide is another typical chemical constituent of this plant. Pharmacology studies have shown that the plant possesses wound healing, antimicrobial, anticancer, antioxidative, and antiviral activities. This review aims to provide the latest and comprehensive information on chemical constituents, pharmacological activities, and quality control of B. striata and to identify future research needs.
Keywords: Bletilla striata, Orchidaceae, chemical constituents, pharmacology, quality control
|How to cite this article:|
Jiang S, Wang MY, Yuan HW, Xie Q, Liu Y, Li BS, Jian YQ, Liu CX, Lou HY, AU, Pan WD, Wang W. Medicinal plant of Bletilla striata: A review of its chemical constituents, pharmacological activities, and quality control. World J Tradit Chin Med 2020;6:393-407
|How to cite this URL:|
Jiang S, Wang MY, Yuan HW, Xie Q, Liu Y, Li BS, Jian YQ, Liu CX, Lou HY, AU, Pan WD, Wang W. Medicinal plant of Bletilla striata: A review of its chemical constituents, pharmacological activities, and quality control. World J Tradit Chin Med [serial online] 2020 [cited 2021 Aug 2];6:393-407. Available from: https://www.wjtcm.net/text.asp?2020/6/4/393/303584
| Introduction|| |
Bletilla striata (Thunb.) Rchb. f. (Orchidaceae) is a perennial herb and mainly distributed in China, Japan, Korea, Thailand, and other Southeast Asian Nations. The tubers of B. striata (“Bai-Ji ” in Chinese) have been used in China for thousands of years, which was initially recorded in ancient book, Shennong's Classic of Materia Medica (“Shen Nong Ben Cao Jing,”. Bletillae Rhizoma (the dried tubers of B. striata) is bitter, pucker, and sweet in flavor and slightly cool and astringent in nature. It is used in Traditional Chinese Medicine (TCM) for the treatment of pulmonary and gastric hemorrhages, chapped skin, ulcerative carbuncle, colds, and burns., Besides, B. striata as a vascular embolizing agent for treating primary hepatic carcinoma has been reported to be more valid than conventional gel foam.,
Even though B. striata was used earlier in China, the study of chemical composition was first started in Japan. At the end of the 20th century, Yamaki, Bai, and Takagi have published more than 10 papers on B. striata in the phytochemistry. Subsequently, other Asian researchers followed and deepened this subject. Today, the plant is known to produce more than 190 compounds, including bibenzyls, phenanthrene derivatives, triterpenoids, steroids, malic acid derivatives, and other compounds. Furthermore, B. striata is also a rich source of polysaccharides, which was not only used in food industry but also applied in medicinal as wound dressing and drug delivery. Among them, bibenzyls, phenanthrene derivatives, and polysaccharides are the typical chemical constituents of this plant. Moreover, pharmacology experiments and clinical practice have demonstrated that its active compounds possess a wide range pharmacological activities, including antibacterial, antioxidant, anti-inflammatory, anticancer, and antiaging effects. Currently, a number of domestic pharmaceutical enterprises in China have been approved to produce B. striata proprietary medicines, including B. striata syrup, B. striata tablets, B. striata granule, and B. striata capsule. The clinical application is extensive. The present paper aimed to review phytochemistry, pharmacological activities, and quality control of B. striata. This information might be useful in designing future studies and in developing new pharmaceuticals containing B. striata or its active ingredients.
| Chemical Constituents|| |
B. striata is a rich source of bibenzyls and phenanthrene derivatives. To date, 44 bibenzyls, 73 phenanthrene derivatives, 8 triterpenoids, 16 steroids, 13 malic acid derivatives, 15 simple phenols, and a number of compounds representing a middle spectrum of constituent classes have been isolated and identified from the plant. Herein, their names, associated references, and structures are shown in [Table 1], [Table 2], [Table 3], [Table 4], [Table 5] and [Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5], [Figure 6], [Figure 7], [Figure 8].
|Figure 2: Structures of dihydrophenanthrenes (45–76) isolated from Bletilla striata|
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|Figure 3: Structures of monomeric phenanthrenes, phenanthraquinones, and triphenanthrenes (77–100) isolated from Bletilla striata|
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|Figure 4: Structures of biphenanthrenes (101–117) isolated from Bletilla striata|
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|Figure 5: Structures of triterpenoids (118–125) isolated from Bletilla striata|
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|Figure 6: Structures of steroids (126–141) isolated from Bletilla striata|
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|Figure 7: Structures of malic acid derivatives (142–154) isolated from Bletilla striata|
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|Figure 8: Structures of simple phenols and other compounds (155–192) isolated from Bletilla striata|
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Bibenzyl is one of the representative chemotypes of B. striata. Dihydro-m-coumaroyl-CoA, with three molecules of malonyl-CoA, is used in bibenzyl biosynthesis. The skeleton of bibenzyls is simple, but their various structural types are led by the amounts and the substituted positions of the methoxy group, the hydroxyl group, and the p-hydroxybenzyl group. A total of 44 bibenzyls with flexible structures have been identified. They can be arranged in Group I – simple bibenzyls (1–11), Group II – benzyl bibenzyls (12–37), and Group III – chiral bibenzyls (38–44). The Group III – chiral bibenzyls have been isolated from B. striata for the first time.
Phenanthrenes are a rather unusual class of aromatic metabolites in nature. It is also another typical chemical constituent of B. striata. Dihydrophenanthrenes (45–76), monomeric phenanthrenes (77–96), biphenanthrenes (101–117), phenanthraquinones (97–99), and triphenanthrenes (100) make up these compounds that have been reported in B. striata. The methoxy and hydroxy groups are main substituents of dihydrophenanthrenes and monomeric phenanthrenes isolated from B. striata. These two moieties number are between 3 and 5 and can generally be found on C-2, C-3, C-4, C-7, or C-8. There structures are shown in [Figure 2], [Figure 3], [Figure 4].
Triterpenoids are extensive in many plants. Only eight triterpenoids have been isolated from B. striata. Among them, there are one saponin of oleanolic acid (125) and seven cycloartane triterpenoids, including four C-32 cycloartane triterpenoids (118–121) and two C-31 cycloartane triterpenoids (122 and 123). Their structures (118–125) are shown in [Figure 5].
Steroids are the one of the secondary metabolites and also widely found in plants. Steroids from B. striata have diverse structures and different substituent groups. Seven steroids (126–129, 131, 133, and 135) and nine saponins of them (130, 132, 134, and 136–141) have been isolated from B. striata, as shown in [Figure 6].
Malic acid derivatives
Malic acid exists in almost all fruits, most of which are kernel fruits. Thirteen malic acid derivatives (142–154) are presented in the tubers and fibrous roots of B. striata. Militarine (146) is abundant in this plant. Their structures are shown in [Figure 7].
B. striata contains so many other compounds such as simple phenolics (155–160, 163–168), anthraquinones (171, 172), lignans (174–177), glycoside compounds (178–184), and anthocyanidins (188–192), whose structures are shown in [Figure 8].
| Biological Activity|| |
B. striata has been used to treat hematemesis, tuberculosis, malignant ulcers, hemorrhoids, traumatic bleeding, and chapped skin. Several extracts and isolated compounds have been evaluated for their wound-healing, antibacterial, antioxidant, anti-inflammatory, anticancer, antiaging, and cytotoxicity activities. In addition, hence, many researchers consider that B. striata polysaccharide (BSP) was the major active component responsible for the various pharmacological activities. Consequently, B. striata has enormous potential for research and exploitation.
Wound healing, as a normal biological process in the animal, contained four main phases: hemostasis, inflammation, proliferation, and resolution. At the first hemostasis stage, the tubers of B. striata have been widely used to cure hematemesis, pulmonary hemorrhage, gastrorrhagia, and traumatic bleeding because it is capable of restraining leakage of blood and stopping bleeding. B. striata micron particles (350–250 μm) enable to improve blood/particle aggregation and to form rapid sealants on wound surfaces to achieve rapid hemostasis. Generally, BSP has been recognized as a major compound in wound healing. BSP has been serving as a wound treatment agent for fairly long period because of its inherent advantage as natural polysaccharides promoting nonspecific activation of the immune system by activating the function of macrophages to clean up the wound site after injury., BSP can be used as wound dress (BSP hydrogel, BSP fibers, and BSP cotton gauze) to treat bleeding.,, Throughout the available literature, nonpolysaccharide components may also have hemostatic effects. It has been reported that five spirostane steroidal saponins (137–141) isolated from the roots of B. striata, all exhibited potent hemostatic activities as shown in significantly decreasing the whole blood clotting time. Some studies showed that the 80% ethanol extract of B. striatahas hemostatic effect which may be related to promote the activation of platelet and coagulation–fibrinolytic system. The wound-healing activities of various extracts (petroleum ether, ethyl acetate, n-butyl alcohol, and water) of B. striata were tested, and water and n-butyl alcohol fractions could significantly increase the maximum platelet aggregation rate.
At the second inflammation phase, nitric oxide (NO), tumor necrosis factor alpha (TNF-α), and interleukin 1 beta (IL-1β) have play important roles in immune and inflammatory responses. Studies reported that therapy with BSP induces coordinate changes in inducible NO synthase (iNOS), TNF-α, and IL-1β mRNA levels, enhances the expression of these cytokines, but has no effect on interferon gamma (IFN-γ) level. IFN-γ is a regulator of hematopoietic stem cells (HSCs) during homeostasis and under conditions of infectious stress. The effective fraction from water or organic extracts of B. striata (collected and analyzed by chromatographic system and inductively coupled plasma mass spectrometer) can reduce the inflammatory cytokine production. Besides, ethyl acetate fraction from methanol extract of B. striata not only reduced NO production but also inhibited iNOS and cyclooxygenase-2 (Cox-2) production. Furthermore, five spirostane steroidal saponins (137–141) isolated from the roots of B. striata displayed selective inhibition of Cox-2 (>90%).
The third proliferative phase commonly follows and overlaps with the inflammatory phase, and it is characterized by epithelial proliferation and migration over the provisional matrix within the wound. In this period, BSP could increase in the expression of the vascular endothelial growth factor (EGF), induce human umbilical vascular endothelial cells' proliferation and migration, and further promote healing by wound contraction., Furthermore, some BSP-based wound dressings such as BSP hydrogel can elevate higher expression of EGF, promote re-epithelialization and collagen deposition by means of TGF-β/Smad signal pathway activation, and then control the inflammatory responses.,
At the last phase, B. striata may have some positive effect on remodeling. However, there is no present literature involving the further mechanism and clear explanations. Except for BSP, some simple phenolics in B. striata can improve the function of wound healing.
Due to the emergence and spread of bacterial resistance and the lack of new antimicrobial drug development, infections caused by multidrug-resistant bacteria have become an increasingly serious problem. Traditional antibiotic screening methods have not kept pace with the evolution of bacterial resistance. The mechanism of phytochemical constituents is different from that of traditional antibiotics, so it has become an international research hotspot. B. striata is one of many Chinese herbal plants with antibacterial activity. Some compounds such as bibenzyls, phenanthrenes, and biphenanthrenes isolated from B. striata are major antimicrobial constituents to against Gram-positive and Gram-negative strains such as Staphylococcus aureus, Bacillus cereus, Staphylococcus epidermidis, Bacillus subtilis, Enterococcus faecalis, and methicillin-resistant S. aureus. Some bibenzyls such as bulbocol (16), shancigusin C (20), shanciguol (29), bletistrin G (31), bletistrin J (34), and bletistrin F (40) showed potent inhibitory activities, with MIC values ranging from 3 to 26 μg/mL against S. aureus ATCC 6538, B. cereus ATCC 6051, and methicillin-resistant S. aureus ATCC 43300. Some phenanthrenes such as 2,7-dihydroxy-4-methoxy-9,10-dihydrophenanthrene (46), 4,5-dihydroxy-2-methoxy-9,10-dihydrophenanthrene (47), and 2-hydroxy-4,7-methoxy-phenanthrene (87) showed moderate inhibitory activities, with MIC of 8–64 μg/mL against S. aureus ATCC 29213, B. subtilis CGMCC 1.1470, E. faecalis ATCC 29212, and S. epidermidis CMCC 26069. Some biphenanthrenes also showed strong inhibitory properties and part of which inhibited effect even more than positive control drugs. For example, 1,1'-linked biphenanthrenes, 4, 7, 4'-trimethoxy-9',10'-dihydro (1,1'-biphenanthrene)-2,2',7'-triol (105) showed strong inhibitory activities, with MIC values ranging from 2 to 4 μg/mL against S. aureus ATCC 29213, S. aureus ATCC 43300, S. epidermidis CMCC 26069, and B. subtilis CGMCC 1.1470 (all the MIC of ampicillin is 8 μg/mL).The biological results are shown in [Table 6].
|Table 6: The antibacterial activities of some compounds from Bletilla striata|
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Apart from the secondary metabolites stated above, ethyl acetate fraction obtained from 95% ethanol extract of B. striata has higher inhibitory activity with MIC values ranging from 0.065 to 1.042 mg/mL against Gram-positive. The phenanthrene fraction (EF60) isolated from the ethanol extract of the fibrous roots of B. striata showed moderate inhibitory activity with MIC values ranging from 8 to 64 μg/mL against S. aureus and B. subtilis. In addition, BSP also had an obvious bacteriostatic effect against S. aureus.
In 1979, Pettit et al. has found that a series of active phenanthrenes, dihydrophenanthrenes, and stilbenes isolated from Combretum caffrum which can inhibit cancer cell growth., Stilbenes isolated from B. striata also contain the same effect, such as 2',6'-bis (4-hydroxybenzyl)-5-methoxybibenzyl-3,3'-diol (26), 2,7-dihydroxy-l, 3-bis(p-hydroxybenzyl)-4-methoxy-9,10-dihydrophenanthrene (60), and 2,7-dihydroxy-1-(p-hydroxybenzyl)-4,8-dimethoxyphenanthrene (91), with an IC50 of 3 μM, sensitized K562/breast cancer resistance protein (BCRP) cells to SN-38 (an active metabolite of CPT-11) by inhibiting BCRP function. Two novel phenanthraquinones, 7-hydroxy-2-methoxyphenanthrene-3,4-dione (97) and 3',7',7-trihydroxy-2,2',4'-trimethoxy- [1,8'-biphenanthrene]-3,4-dione (98), could block the G1-to-S phase transition process and then display significant inhibitory effect against the human breast cancer cells (MCF-7), HT-29, nonsmall cell lung adenocarcinoma (A549), and HUVEC cell lines, with IC50 values ranging from 12.46 ± 2.71 to 48.35 ± 3.87 μg/mL. Some bibenzyls such as 3',4''-dihydroxy-5',3'',5''-trimethoxybibenzyl (3), batatasin III (5), and gigantol (7) showed considerable cytotoxicity against A549, ovarian cancer cells (SKOV-3), skin melanoma (SK-MEL-2), and colon cancer cells (HCT15) cells with IC50 values of 5.28–24.51 μM. In addition, five spirostane steroidal saponins (137–141) displayed significant cytotoxicity against lung cancer cells (A-549), human gastric carcinoma cells (BGC-823), human hepatocellular carcinoma cells (HepG2), human myeloid leukemia (HL-60), MCF-7, hepatocellular carcinoma cells (SMMC-7721), and colon cancer cells (W480) with IC50 values <30 μM. Oleanolic acid 3-O-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside (125) also exhibited antiproliferative activity against the MCF-7, HT-29, and A-549 cells and could induce G0/G1 phase arrest effectively.The biological results are shown in [Table 7].
|Table 7: The anticancer activities of some compounds from Bletilla striata|
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Furthermore, the chloroform subfraction of the fibrous root of B. striata can induce HepG2 cells apoptosis, which implied cancer therapeutic effect. BSP is also illustrated to have antitumor activity. BSP-1 (a backbone of 1→4-linked β-D-mannose and glucose residues) could inhibit the tumor proliferation of HepG2-bearing mice. Recently, more and more studies demonstrated that BSP was a promising drug carrier due to its brilliant biocompatibility, easy availability, and low toxicity. Silymarin was loaded into the nanoassembly of BSP conjugates with stearic acid (SA), which can improve cytotoxicity and cell uptake in HepG2 cell lines in vitro. BSP to conjugate alendronate (ALN-BSP) demonstrated high efficiency in targeting and eliminating tumor-associated macrophages, which accordingly reduced angiogenesis, reactivated immune surveillance, and suppressed tumor growth in vivo. The anticancer activity of docetaxel (DTX)-based SA-modified BSPs copolymer micelles against HepG2, HeLa, MCF-7, and SW-480 cells was superior to that of DTX injection.
Many studies have been performed to search for novel antioxidants instead of tocopherols in plant materials, such as spices, oil seeds, and polysaccharides. BSP has obvious antioxidant effect which was proved by hydroxyl radical scavenging assay, superoxide anion radical-scavenging assay, DPPH-free radical scavenging activity, and chelation of ferrous ions., BSP film can reduce the rate of water loss in cherry tomato, inhibit its respiratory intensity, and delay the aging process of the fruit. In addition, the sch of B. striata has strong DPPH radical scavenging activity, ferric-reducing antioxidant activity, and tyrosinase inhibitory activity because of the total phenolic contents. It is also worth indicating that microorganism fermentation could further build the antioxidative activity of B. striata. For instance, Fusarium avenaceum and Fusarium oxysporum fermentation enhances the total phenolic contents as well as the antioxidant activities of B. striata.
The influenza virus is a branch of the virus. At present, the main measures to prevent and treat influenza are vaccination and treatment drugs. Because of the rapid transmission of influenza virus and the easy occurrence of antigenic variation, the existing vaccines cannot fight against new virus infection, while the development of new specific vaccines has obvious lag, which cannot effectively prevent the outbreak and epidemic of influenza. Therefore, it is important to find new influenza antiviral drugs, especially from natural products. In Madin–Darby canine kidney models, 2-hydroxy-4,7-methoxyphenanthrene (87) and 1,1'-linked biphenanthrenes, 4, 7, 4'-trimethoxy-9',10'-dihydro (1,1'-biphenanthrene)-2,2',7'-triol (105) showed inhibitory activities against influenza virus A/Sydney/5/97 (H3N2) with IC50 values of 43.3 ± 5.3 and 28.6 ± 4.3 μM, respectively. Furthermore, water and 95% ethanol extracts of B. striata play an anti-influenza virus effect by inhibiting virus HA receptor and intervening the viral RNA synthesis and neuraminidase activity.
| Quality Control|| |
The quality of the medicinal materials is closely related to the characteristics of regional, diversity, variability, and humanity. Due to the complex situation of Chinese medicinal materials, such as multiple sources and producing areas, the quality of pharmaceutical products varies greatly, especially the quantity of active ingredients. Hence, Liu et al. proposed a new concept on Chinese medicine quality markers (Q-Marker) which can be a reflection of Chinese medicine safety and effective labeling substances for quality control. Q-Marker is a chemical substance that is inherent in or formed during the processing and preparation of TCM and its products, and it is closely related to the functional properties of TCM. In China, Guizhou province has the largest output and the best quality of B striata. Zhengan County in this province is accepted as the authentic region to produce the crude B. striata. This plant is as an important material of Chinese medicine; definite quality control can offer help for its effects on human health. However, the Q-Marker and potential mechanisms for producing genuine medicinal materials are not fully clear. As the main method of quality control of medicinal materials, B. striata has no uniform quality standard of medicinal ingredients. The Chinese Pharmacopoeia (2015) recommends identifying the authentic properties of B. striata according to the morphologic, microscopic, and thin-layer chromatography approaches and by ensuring that the total ash points are ≤5.0%., Even so, there are still some studies reporting on its quality control. BSP is one of the main active components of the plant, and its content is an important quality evaluation of B. striata. Wang et al. compared the content of polysaccharides in cultivated and wild B. striata and found that the content in cultivated plant was stable, while the content in wild plant was labile. There were also significant differences in the content of BSP in different production areas. Zhou et al. used a HPLC method to find that the average contents of militarine (146) and gastrodin (178) of wild B. striata were higher than that of cultivated B. striata. Phenols are another important active component of B. striata. Chi et al. used nine phenols as reference substances to find that the active ingredients of B. striata were correlated with the elevation. Furthermore, medicinal parts also will affect the active content of B. striata. For example, the content of polysaccharides in the tubers of B. striata is higher than the fibrous roots; the total phenols content in the fibrous roots of B. striata is higher than the tubers. Hence, the polysaccharides and the total phenols may be used as the Q-Markers for the quality control of B. striata. In addition, cultivation style, production area, elevation, and medicinal parts all affect the active content of B. striata. To sum up, its needs more experiments to verify quality control of B. striata.
| Conclusion|| |
As an ancient Chinese medicine, B. striata can cure various diseases such as pulmonary and gastric hemorrhages, chapped skin, and ulcerative carbuncle. The plant contains many types of chemical constituents, including bibenzyls, phenanthrene derivatives, malic acid derivatives, steroids, and polysaccharides. These chemical constituents are the basis of substances that have a variety of biological functions such as hemostasis, anti-inflammatory, antibacterial, anticancer, antioxidant, and antiviral activities. BSP has been considered as the main active component of the plant. It not only has been exploited as useful wound dressings, but it also has been demonstrated as a promising drug carrier. However, the bioavailability of BSP in body and property evaluation for the best therapeutic function is needed for deep investigations. Thus, it is necessary to find main active compounds for wound healing with B. striata by advanced chemical and pharmacological studies. Furthermore, Bletillae Rhizoma mainly depends on wild resources, but excessive exploitation has led to the sharp shrinkage of B. striata wild resources and the loss of genetic diversity in China. Future directions of researches will be focused on cultivation, biological activities, and underlying mechanisms of B. striata. Hence, the research on breeding, cultivation and quality control of this plant is also a lot every year. This review summarized the latest chemical constituents and pharmacological activities of B. striata and tried to illuminate their connections and hope to provide further research on this medicinal plant which has great development potential.
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Conflicts of interest
There are no conflicts of interest.
| References|| |
Feng JQ, Zhang RJ, Zhao WM. Novel bibenzyl derivatives from the tubers of Bletilla striata
. Helv Chim Acta 2008;91:520-5.
Tang YF, Ruan CF, Ying C, Zhang HW. Research progress on chemical constituents and medical functions in plants of Bletilla
Rchb. Chin Tradit Herbal Drugs 2014;45:2864-72.
Hossain MM. Therapeutic orchids: Traditional uses and recent advances--An overview. Fitoterapia 2011;82:102-40.
Wang Y, Liu D, Chen S, Wang Y, Jiang H, Yin H. A new glucomannan from Bletilla striata
: structural and anti-fibrosis effects. Fitoterapia 2014;92:72-8.
Liu BS, Huang TB. A novel wound dressing composed of nonwoven fabric coated with chitosan and herbal extract membrane for wound healing. Polym Composite 2010;31:1037-46.
Wang LN, He YZ, Zhao QD, Deng YR, Wu PQ, Zhang YJ. Phenolic compounds from Bletilla striata
. J Asian Nat Prod Res 2017;19:981-6.
Xu DL, Pan YC, Chen JS. A review on chemical constituents, pharmacological properties and clinical application of Bletilla striata
. Front Pharm 2019;10:1-19.
Chen Z, Cheng L, He Y, Wei X. Extraction, characterization, utilization as wound dressing and drug delivery of Bletilla striata
polysaccharide: A review. Int J Biol Macromol 2018;120:2076-85.
Wang W, Meng H. Cytotoxic, anti-inflammatory and hemostatic spirostane-steroidal saponins from the ethanol extract of the roots of Bletilla striata
. Fitoterapia 2015;101:12-8.
Sun AJ, Pang SQ, Wang GQ. Advances of chemical constituents and pharmacological activities of Bletilla striata
. Glob Traditi Chin Med 2016;9:507-11.
Reinecke T, Kindl H. Characterization of bibenzyl synthase catalysing the biosynthesis of phytoalexins of orchids. Phytochemistry 1993;35:63-6.
Yamaki M, Kato T, Bai L, Inoue K, Takagi S. Methylated stilbenoids from Bletilla striata
. Phytochemistry 1991;30:2759-60.
Woo KW, Park JE, Choi SU, Kim KH, Lee KR. Phytochemical constituents of Bletilla striata
and their cytotoxic activity. Nat Prod Sci 2014;20:91-4.
Jiang S, Chen CF, Ma XP, Wang MY, Wang W, Xia Y, et al
. Antibacterial stilbenes from the tubers of Bletilla striata
. Fitoterapia 2019;138:104350.
Yamaki M, Bai L, Inoue K, Takagi S. Biphenanthrenes from Bletilla striata
. Phytochemistry 1989;28:3503-5.
Jiang S, Yang J, Song ZQ, Lou HY, Ma XP, Wu MK, et al
. Study on chemical constituents of Bletilla striata
. Nat Prod Res Dev 2017;29:230-3.
Han GX, Wang LX, Zhang WD, Yang Z, Li TZ, Jiang T, et al
. Study on chemical constituents of Bletilla striata
(I). Aca J Second Military Med Unvi 2002;23:443-5.
Ma XJ, Cui BS, Han SW, Li S.Chemical constituents from tuber of Bletilla striata
. Zhongguo Zhong Yao Za Zhi 2017;42:1578-84.
Jiang S, Wan K, Lou HY, Yi P, Zhang N, Zhou M, et al
. Antibacterial bibenzyl derivatives from the tubers of Bletilla striata
. Phytochemistry 2019;162:216-23.
Bai L, Kato T, Inoue K, Yamaki M, Takagi S. Stilbenoids from Bletilla striata
. Phytochemistry 1993;33:1481-3.
Xu DL, Pan YC, Li L, ShangGuan YN, Zhang SB, Liu GY, et al
. Chemical constituents of Bletilla striata
. J Asian Nat Prod Res 2019;21:1184-9.
Takagi S. Antimicrobial agents from Bletilla striata
. Phytochemistry 1983;22:1011-5.
Han GX. Wang LX. Gu ZB. Zhang WD. A new bibenzyl derivative from Bletilla striata
. Acta Pharm Sin 2002;37:194-5.
Yu HS, Dai BL, Qian CD, Ding ZS, Jiang FS, Jin B, et al
.Antibacterial activity of chemical constituents isolated from fibrous roots of Bletilla striata
. Zhong Yao Cai 2016;39:544-7.
Zhou D, Chen G, Ma YP, Wang CG, Lin B, Yang YQ, et al
. Isolation, structural elucidation, optical resolution, and antineuroinflammatory activity of phenanthrene and 9,10-dihydrophenanthrene derivatives from Bletilla striata
. J Nat Prod 2019;82:2238-45.
Yamaki M, Kato T, Bai L, Inoue K, Takagi S. Phenanthrene glucosides from Bletilla striata
. Phytochemistry 1993;34:535-7.
Yamaki M, Bai L, Inoue K, Takagi S. Benzylphenanthrenes from Bletilla striata
. Phytochemistry 1990;29:2285-7.
Yamaki M, Bai L, Kato T, Inoue K, Takagi S. Three dihydrophenanthropyrans from Bletilla striata
. Phytochemistry 1993;32:427-30.
Kang YY, Tu YB, Zhu C, Meng XF, Yan Y, Wu CH, et al
. Two new stilbenoids from Bletilla striata
. J Asian Nat Prod Res 2019;21:1170-6.
Xiao SJ, Xu DL, Zhang MS. A novel phenanthrene-1,2-dione from Bletilla striata
. Chin J Org Chem 2016;36:638-41.
Morita H, Koyama K, Sugimoto Y, Kobayashi J. Antimitotic activity and reversal of breast cancer resistance protein-mediated drug resistance by stilbenoids from Bletilla striata
. Bioorg Med Chem Lett 2005;15:1051-4.
Xiao S, Yuan FM, Zhang MS, Yu SY, Li JD, Yi XD, et al
. Three new 1-(p-hydroxybenzyl) phenanthrenes from Bletilla striata
. J Asian Nat Prod Res 2017;19:140-4.
Bai L, Kato T, Inoue K, Yamaki M, Takagi S. Blestrianol A, B and C, biphenanthrenes from Bletilla striata
. Phytochemistry 1991;30:2733-5.
Yamaki M, Bai L, Kato T, Inoue K, Takagi S, Yamagata Y, et al
. Blespirol, a phenanthrene with a spirolactone ring from Bletilla striata
. Phytochemistry 1993;33:1497-8.
Sun A, Liu J, Pang S, Lin J, Xu R. Two novel phenanthraquinones with anti-cancer activity isolated from Bletilla striata
. Bioorg Med Chem Lett 2016;26:2375-9.
Bae JY, Lee JW, Jin Q, Jang H, Lee D, Kim Y, et al
. Chemical constituents isolated from Bletilla striata
and their inhibitory effects on nitric oxide production in RAW 264.7 Cells. Chem Biodivers 2017;14:e1600243.
Qian CD, Jiang FS, Yu HS, Shen Y, Fu YH, Cheng DQ, et al
. Antibacterial Biphenanthrenes from the fibrous roots of Bletilla striata
. J Nat Prod 2015;78:939-43.
Bai L, Yamaki M, Inoue K, Takago S. Blestrin A and B, bis (dihydrophenanthrene) ethers from Bletilla striata
. Phytochemistry 1990;29:1259-60.
Yamaki M, Bai L, Kato T, Inoue K, Takagi S, Yamagata Y, et al
. Bisphenanthrene ethers from Bletilla striata
. Phytochemistry 1992;31:3985-7.
Yang L, Peng C, Meng CW, He CJ, Li XH, Guo L, et al
. A new macrolide and six cycloartane triterpenoids from the tubers of Bletilla striata
. Biochem Syst Eco 2014;57:238-41.
Yamaki M, Honda C, Kato T, Bai L, Takagi S. The steroids and triterpenoids from Bletilla striata
. Nat Med 1997;51:493-6.
Sun AJ, Pang SQ, Wang GQ. Chemical constituents from Bletilla striata
and their anti-tumor activities. Chin Pharm J 2016;51:101-4.
Han GX, Wang LX, Wang ML, Zhang WD. Studies on the chemical constituents of Bletilla striata
. J Pharm Pract 2001;19:360-1.
Sun AJ, Pang SQ, Wang GQ. Separation of chemical constituents from Bletilla striata
and their antitumor activities. Chin Pharmacol J 2016;47:35-8.
Park JE, Woo KW, Choi SU, Lee JH, Lee KR. Two new cytotoxic spirostane-steroidal saponins from the roots of Bletilla striata
. Helv Chim Acta 2014;97:56-63.
Guan HY, Yan Y, Wang YL, Wang AM, Liu JH, He X, et al
. Isolation and characterization of two new 2-isobutylmalates from Bletilla striata
. Chin J Nat Med 2016;14:871-5.
Sakuno E, Kamo T, Takemura T, Sugie H, Hiradate S, Fujii Y. Contribution of militarine and dactylorhin A to the plant growth-inhibitory activity of a weed-suppressing orchid, Bletilla striata
. Weed Biol Manag 2010;10:202-7.
Mei C, Xiang W, Yang W, Huang Y, Wang Y, Wang A. Simultaneous determination of six components in Bletilla striata
by UPLC-MS/MS. Nat Prod Res Develop 2016;28:1233-7.
Yan Y, Guan HY, Wang AM, Wang YL, Li YJ, Liao SG. Chemical constituents of Bletillae rhizoma. Chin J Exp Tradit Med Form 2014;20:57-60.
Song Y, Zeng R, Hu L, Maffucci KG, Ren X, Qu Y. In vivo
wound healing and In vitro
antioxidant activities of Bletilla striata
phenolic extracts. Biomed Pharmacother 2017;93:451-61.
Chen L, Liu CX, He XL, Zhang XH. Simultaneous determination of militarine, protocatechuic acid and caffeic acid in Bletilla striata
by LC-MS/MS. Chin Pharm 2015;2:230-2.
Dai O, Yang L, Zhou QM, Peng C. Chemical constituents from tubers of Bletilla striata
. Chin J Exp Tradit Med Form 2018;24:43-7.
Wu TY, Chen CC, Lay HL. Study on the components and antioxidant activity of the Bletilla
plant in Taiwan. J Food Drug Anal 2010;18:279-89.
Wang L, Han G, Shu Y, Liu W, Zhang W. Studies on chemical constituents of Bletilla striata
(Thunb) Reichb. f. Chin J Chin Mater Med 2001;26:690-2.
Chen CF, Jiang S, Lou HY, Wan K, Ma XP, Wu MK, et al
. Glycoside constituents from Bletilla striata
. Chin Tradit Herbal Drugs 2019;50:4879-83.
Tatsuzawa F, Saito N, Shigihara A, Honda T, Toki K, Shinoda K, et al
. An acylated cyanidin 3,7-diglucoside in the bluish flowers of Bletilla striata
'Murasaki Shikibu' (Orchidaceae
). J Jpn Soc for Hortic Sci 2010;79:215-20.
Saito N, Ku M, Tatsuzawa F, Lu TS, Yokoi M, Shigihara A, et al
. Acylated cyanidin glycosides in the purple-red flowers of Bletilla striata
. Phytochemistry 1995;40:1523-9.
Gosain A, DiPietro LA. Aging and wound healing. World J Surg 2004;28:321-6.
He X, Wang X, Fang J, Zhao Z, Huang L, Guo H, et al
. Bletilla striata
: Medicinal uses, phytochemistry and pharmacological activities. J Ethnopharmacol 2017;195:20-38.
Zhang C, Zeng R, Liao Z, Fu C, Luo H, Yang H, et al
. Bletilla striata
micron particles function as a hemostatic agent by promoting rapid blood aggregation. Evid Based Complement Alternat Med 2017;2017:582405.
Süntar I, Akkol EK, Nahar L, Sarker SD Wound healing and antioxidant properties: do they coexist in plants? Free Rad Antiox 2012;2:1-7.
Aduba DC, Yang H. Polysaccharide fabrication platforms and biocompatibility assessment as candidate wound dressing materials. Bioengineering (Basel) 2017;4:1-16.
Cui X, Zhang X, Yang Y, Wang C, Zhang C, Peng G. Preparation and evaluation of novel hydrogel based on polysaccharide isolated from Bletilla striata
. Pharm Dev Technol 2017;22:1001-11.
Xiang Y, Ye Q, Li W, Xu W, Yang H. Preparation of wet-spun polysaccharide fibers from Chinese medicinal Bletilla striata
. Mater Lett 2014;117:208-10.
Venkatrajah B. Biopolymer and Bletilla striata
herbal extract coated cotton. J Med Sci 2012;12:148-60.
Zhao FF, Yang X, Xu D, Dong L. Hemostatic effect and mechanism of a non-polysaccharide fraction of Bletilla striata
. Chin Pharm Bull 2016;32:1121-6.
Lu B, Xu Y, Zhang H, Li T, Qiu Y. Effects of different extracts from Bletilla
colloid on rabbit platelet aggregation. Med Pharm J Chin PLA 2005;21:330-2.
Diao H, Li X, Chen J, Luo Y, Chen X, Dong L, et al
. Bletilla striata
polysaccharide stimulates inducible nitric oxide synthase and proinflammatory cytokine expression in macrophages. J Biosci Bioeng 2008;105:85-9.
Baldridge MT, King KY, Boles NC, Weksberg DC, Goodell MA. Quiescent haematopoietic stem cells are activated by IFN-γ in response to chronic infection. Nature 2010;465:793-7.
Zu YY, Liu QF, Tian SX, Jin LX, Jiang FS, Li MY, et al
. Effective fraction of Bletilla striata
reduces the inflammatory cytokine production induced by water and organic extracts of airborne fine particulate matter (PM 2.5) In vitro
. BMC Complem Al Tern M 2019;19:1-12.
Yoon JH, Park SG, Lee MJ, Park JY, Seo KS, Woo KC, et al
. Antioxidant and anti-inflammatory effects of Bletilla striata
Reichenbach fil. fractions as cosmetic. J Life Sci 2013;23:1073-8.
Guo S, Dipietro LA. Factors affecting wound healing. J Dent Res 2010;89:219-29.
Luo Y, Diao H, Xia S, Dong L, Chen J, Zhang J. A physiologically active polysaccharide hydrogel promotes wound healing. J Biomed Mater Res A 2010;94:193-204.
Wang C, Sun J, Luo Y, Xue W, Diao H, Dong L, et al
. A polysaccharide isolated from the medicinal herb Bletilla striata
induces endothelial cells proliferation and vascular endothelial growth factor expression in vitro. Biotechnol Lett 2006;28:539-43.
Zhang C, He Y, Chen Z, Shi J, Qu Y, Zhang J. Effect of polysaccharides from Bletilla striata
on the healing of dermal wounds in mice. Evid Based Complement Alternat Med 2019;2019:9212314.
Peng F, Wan F, Xiong L, Peng C. Research on antibacterial activity and substance of Bletilla striata
(Thund.) Reichb. f Lishizhen Med Mate Med Res 2013;24:1061-3.
Guo JJ, Dai BL, Chen NP, Jin LX, Jiang FS, Ding ZS, et al
. The anti-Staphylococcus aureus activity of the phenanthrene fraction from fibrous roots of Bletilla striata
. BMC Complement Altern Med 2016;16:491.
Li Q, Li K, Huang SS, Zhang HL, Diao YP. Optimization of extraction process and antibacterial activity of Bletilla striata
Polysaccharides. Asian J Chem 2014;26:3574-80.
Pettit GR, Singh SB, Niven ML, Schmidt JM. Cell growth inhibitory dihydrophenanthrene and phenanthrene constituents of the African tree Combretum caffrum. Can J Chem 1988;66:406-13.
Kovács A, Vasas A, Hohmann J. Natural phenanthrenes and their biological activity. Phytochemistry 2008;69:1084-110.
Jiang FS, Li WP, Huang YF, Chen YT, Jin B, Chen NP, et al
. Antioxidant, antityrosinase and antitumor activity comparison: The potential utilization of fibrous root part of Bletilla striata
(Thunb.) Reichb. f. PloS One 2013;8:e58004.
Zhang G, Qiao J, Liu X, Liu Y, Wu J, Huang L, et al
. Interactions of self-assembled Bletilla striata
polysaccharide nanoparticles with bovine serum albumin and biodistribution of its docetaxel-loaded Nanoparticles. Pharmaceutics 2019;11:43-63.
Chen SS, Wu B, Tan T, Xie SS. Isolation, purification and structural characterization of Bletilla striata
polysaccharides and its antitumor activity. Chin Tradit Herbal Drugs 2019;50:1921-6.
Ma Y, He S, Ma X, Hong T, Li Z, Park K, et al
. Silymarin-loaded nanoparticles based on stearic acid-modified Bletilla striata
polysaccharide for hepatic targeting. Molecules 2016;21:265.
Zhan X, Jia L, Niu Y, Qi H, Chen X, Zhang Q, et al
. Targeted depletion of tumour-associated macrophages by an alendronate-glucomannan conjugate for cancer immunotherapy. Biomaterials 2014;35:10046-57.
Guan QX, Sun DD, Zhang GY, Sun C, Wang M, Ji DY, et al
. Docetaxel-loaded self-assembly stearic acid-modified Bletilla striata
polysaccharide micelles and their anticancer effect: Preparation, characterization, cellular uptake and In vitro
evaluation. Molecules 2016;21:1641-56.
Su JD, Osawa T, Namiki M. Screening for antioxidative activity of -‚zcrude drugs. Agr Biol Chem 1986;50:199-203.
Cai JY, Xiong JW, Huang YF, Zhao YY. Study on ultrasonic-microwave extraction technology and antioxidant activity of white and polysaccharide. Sci Technol Food Ind 2016;37:274-8.
Qu Y, Li C, Zhang C, Zeng R, Fu C. Optimization of infrared-assisted extraction of Bletilla striata
polysaccharides based on response surface methodology and their antioxidant activities. Carbohydr Polym 2016;148:345-53.
He HL, Gu GP, Zhang WM. Preservation research on cherry tomato fruits coated with Bletilla Glucomannan (Bg) film. Food Sci 2007;4:336-40.
Dong JW, Cai L, Xiong J, Chen XH, Wang WY, Shen N, et al
. Improving the antioxidant and antibacterial activities of fermented Bletilla striata
with Fusarium avenaceum and Fusarium oxysporum. Process Biochem 2015;50:8-13.
Shi Y, Zhang B, Lu Y, Qian C, Feng Y, Fang L, et al
. Antiviral activity of phenanthrenes from the medicinal plant Bletilla striata
against influenza A virus. BMC Complement Altern Med 2017;17:273.
Zhang B, Shi Y, Zhou FF, Lu YY. Anti-influenza virus activity of extracts from the tubes of Bletilla striata In vitro
. Chin Med Mat 2017;40:2930-5.
Liu CX. Recognizing healthy development of Chinese medicine industry from resources quality-quality markers of Chinese medicine. Chin Tradit Herbal Drugs 2016;47:3149-54.
Liu CX, Chen SL, Xiao XH, Zhang TJ, Hou WB, Liao ML. A new concept on quality marker of Chinese Materia Medica: Quality control for Chinese medicinal products. Chin Tradit Herbal Drugs 2016;47:1443-57.
Wang AM, Wang YL, Zhen L, Li YJ. Content determination of polysaccharides in Bletilla striata
. Chin J Chin Mater Med 2009;34:2963-5.
Qin YD, Wang RB, Zhou Z. Content determination of polysaccharide in Bletilla striata
samples from different regions. J Anhui Sci Technol Univ 2014;28:41-4.
Zhou HT, Chen ZM, Li WB, Quan L, Zhang X, Hu CJ, et al
. Fingerprints establishment of wild and cultivated Bletilla striata
and content determination of gastrodin and militarine. Chin Med Mat 2018;41:2527-33.
Chi MY, Huang Y, Li YJ, Wang AM. Correlation between altitude and nine kinds of main components in Bletilla Rhizoma from Guizhou Province. Chin J Exp Tradit Med Form 2016;22:36-9.
Yu HS, Shi ZZ, Lv D, Pan P, Qian CD, Chen JZ, et al
. Comparative study of polysaccharide in fibrous root and tuber of Bletilla striata
. J Yunnan Univ Tradit Chin Med 2015;38:29-32.
Zhou YK, Li WP, Tian SS, Ma DD, Jiang FS, Ding ZS. Determination of total polyphenol content in different parts of Rhizoma Bletillae striatae. Chin J Exp Tradit Med Form 2012;18:161-4.
Zhang YJ, Sun W, He Y, Wang SF, Wang Q, Yuan F, et al
. Current situation and prospect of resource evaluation and sustainable utilization on Bletilla striata
. Chin J Chin Mater Med 2018;43:4397-403.
[Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5], [Figure 6], [Figure 7], [Figure 8]
[Table 1], [Table 2], [Table 3], [Table 4], [Table 5], [Table 6], [Table 7]
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