• Users Online: 143
  • Print this page
  • Email this page

Table of Contents
Year : 2020  |  Volume : 6  |  Issue : 4  |  Page : 408-422

The fruits of Xanthium sibiricum Patr: A review on phytochemistry, pharmacological activities, and toxicity

1 Key Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin, 150040, PR China
2 School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, 528458, PR China
3 Key Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin, 150040; School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, 528458, PR China

Date of Submission05-May-2020
Date of Acceptance22-May-2020
Date of Web Publication16-Dec-2020

Correspondence Address:
Prof. Hai-Xue Kuang
Key Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin, 150040
PR China
Prof. Qiu-Hong Wang
Key Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin, 150040, PR China.School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, 528458
PR China
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/wjtcm.wjtcm_49_20

Rights and Permissions

In recent years, drug development and research have gradually shifted from chemical synthesis to biopharmaceutical and natural drugs. Natural medicines, such as traditional Chinese medicine, have been among the first studied because of their long medicinal history, simplicity, and the relatively low cost of research. Among them, Xanthii Fructus (XF) is famous for the treatment of sinusitis. In this article, the achievements of research on XF from 1953 to 2020 are systematically reviewed, focusing on the aspects of chemical constituents, pharmacological effects, clinical applications, toxicity and side effects, and processing methods. To date, there have been significant advances in both the phytochemistry and pharmacology of XF. Some traditional uses have been validated and clarified in modern pharmacological studies. However, its mechanism of action in the treatment of allergic diseases has not been satisfactorily explained. Further in vitro and in vivo studies are required to rationally develop new drugs and to elucidate the therapeutic potential of XF. A comprehensive evaluation of XF and an understanding of network pharmacology are also needed.

Keywords: Pharmacological activities, phytochemistry, toxicity, Xanthii Fructus

How to cite this article:
Jiang H, Wang Xj, Yang L, Zhang JX, Hou AJ, Man WJ, Wang S, Yang BY, Chan K, Wang QH, Kuang HX. The fruits of Xanthium sibiricum Patr: A review on phytochemistry, pharmacological activities, and toxicity. World J Tradit Chin Med 2020;6:408-22

How to cite this URL:
Jiang H, Wang Xj, Yang L, Zhang JX, Hou AJ, Man WJ, Wang S, Yang BY, Chan K, Wang QH, Kuang HX. The fruits of Xanthium sibiricum Patr: A review on phytochemistry, pharmacological activities, and toxicity. World J Tradit Chin Med [serial online] 2020 [cited 2021 Jan 25];6:408-22. Available from: https://www.wjtcm.net/text.asp?2020/6/4/408/303539

  Introduction Top

More than 20,000 kinds of plants are used in traditional medicines worldwide. Traditional Chinese medicine (TCM) is famous for its unique curative effects. Xanthii Fructus (XF), or “cangerzi” in Chinese, is widely studied as a traditional Chinese medicinal agent. XF is the dried fruit of the composite plant Xanthium sibiricum Patr. With involucre as shown in [Figure 1]. The fruit ripens in autumn, is harvested and dried, and the impurities such as stems and leaves are removed. XF was first described in Shennong's Classic of Materia Medica (during the Qin and Han Dynasties) and was later recorded in the Compendium of Materia Medica (Ming Dynasty). XF is often used to treat rhinitis, headache due to a cold, limb cramps and numbness, ulcers, and itching.[1] Modern pharmacological research has shown that XF also exerts hypoglycemic, anti-inflammatory, and other effects.[2],[3],[4] Although XF has high efficacy, its toxicity cannot be ignored. Cases of poisoning induced by accidental ingestion of XF are often reported, mainly resulting from drug-induced liver injury.[5] This toxicity limits the clinical use of XF. To reduce the toxicity of XF and improve its therapeutic effects, processed, especially stir-fried, products are often used in China.
Figure 1: The plant Xanthium sibiricum Patr. and Xanthii Fructus

Click here to view

In recent years, there have been many reports on XF. The main chemical constituents of XF are sesquiterpenoid lactone, glycoside, and phenolic acid; it also contains unsaturated fatty acids and other substances.[6],[7],[8],[9],[10],[11] In the 2015 edition of Pharmacopoeia of the People's Republic of China (ChP), carboxyl atractyloside was used as an index to evaluate the toxicity of XF, while chlorogenic acid content was used to evaluate quality. Some methods, such as high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS), can provide a reference for optimizing quality and safety controls of XF.[12],[13]

This article systematically reviews recent studies on the phytochemistry, pharmacology, and toxicology of XF. The purpose was to study XF in depth in order to elucidate its application and pharmacological activity and to discuss the limitations of current studies. Future research directions for XF are considered in order to provide a scientific basis for the development of new drugs and to elucidate its full therapeutic potential.

  Phytochemistry Top

XF contains an abundance of chemical constituents. Increasing research activity has enhanced our understanding of its chemical constituents. Thus far, chemical constituents including phenolic acid, sesquiterpenes, glycosides, thiazine, and lignans have been discovered


Phenylpropanoids are important chemical components in XF, and phenolic acids are the most common phenylpropanoids. There are many phenolic acid compounds, and chlorogenic acid is the most abundant organic acid in XF. Phenolic acid plays an important role in the clinical effects of XF. It has favorable antioxidant, anti-inflammatory, antimicrobial, enzyme inhibitory, liver cell protective, and platelet aggregation inhibitory activities, as well as other biological activities.[14],[15],[16],[17],[18],[19],[20] In addition to chlorogenic acid, other phenolic acids exist in XF, such as neochlorogenic acid, cryptochlorogenic acid, caffeic acid (1), ferulic acid (2), isochlorogenic acid (3), protocatechuic acid (4), and caffeoylquinic acids.[21],[22],[23],[24],[25] Other studies have shown that the phenolic acid content in these medicinal materials is related to factors such as origin, harvest time, processing time, and temperature.[22] The names and chemical structures of other phenylpropanoids are shown in [Table 1] and [Figure 2].
Figure 2: Chemical structure of phenylpropenoids reported from Xanthii Fructus

Click here to view
Table 1: Phenylpropenoids reported from Xanthii Fructus

Click here to view


Glycosides are widely distributed in the roots, stems, leaves, flowers, and fruits of plants.[40] They are mostly colored crystals that dissolve in water.[41] They are generally bitter and some are highly toxic. Sugars and other substances are formed by hydrolysis.[41] The toxic components of XF are mainly water-soluble glycosides.[34] Jiang (2017) isolated and identified the toxic parts based on their selection by pharmacological screening. Experiments have shown that the toxic components of XF are carboxyatractyloside (1), atractyloside (2), and 3',4'-dedisulfated-atractyloside (3).[35],[36],[37] After stir-frying, the content of atractyloside decreases, and the toxicity of XF decreases.[36] The glycosides in XF include hemiterpene glycosides, monoterpene glycosides, and diterpenoid glycosides. Details of the glycoside compounds are shown in [Table 2]. The corresponding chemical structures are shown in [Figure 3].
Figure 3: Chemical structure of glycosides reported from Xanthii Fructus

Click here to view
Table 2: Glycosides reported from Xanthii Fructus

Click here to view


The characteristic components of Compositae are sesquiterpenoid lactones.[45],[46] More than 300 sesquiterpenoid lactones have been isolated from Compositae alone. These compounds have anti-inflammatory, analgesia, antitumor, anti-allergic, antibacterial, antiulcer, and other activities, and they affect the central nervous system and cardiovascular system.[47],[48],[49],[50],[51],[52],[53],[54] Most of the sesquiterpene lactones in XF have been extracted with petroleum ether, methanol, ethanol, chloroform, acetone, and other organic solvents. These organic solvents have been further extracted, removed, and extracted by column chromatography to obtain sesquiterpene lactones.[55] To date, approximately forty sesquiterpene lactones have been identified in XF [Table 3]. The detailed chemical structure is shown in [Figure 4]. Xanthatin is the main active substance of XF, which exerts antitumor effects.[54]
Figure 4: Chemical structure of sesquiterpenoids reported from Xanthii Fructus

Click here to view
Table 3: Sesquiterpenoids reported from Xanthii Fructus

Click here to view


Twenty lignans have been identified in XF since 2017. As shown in [Figure 5]. They include xanthiumnolic B (1), leptolepisol D (2), fructusol A (3), (-)-1-O-β-D-glucopyranosyl-2-{2-methoxy-4-[1-(E)-propen-3-ol] phenoxyl}-propane-3-ol (4), and dihydrode-hydrodiconiferyl alcohol (5); the other components are shown in [Table 4]. Among them, in 2017, xanthiumnolic B was shown to have anti-inflammatory effects.[66]
Figure 5: Chemical structure of lignanoids reported from Xanthii Fructus

Click here to view
Table 4: Lignanoids reported from Xanthii Fructus

Click here to view


Ten thiazide compounds have been reported to date: xanthiazone (1), 2-hydroxy-xanthiazone (2), 7-hydroxymethyl-8,8-dimethy l-4,8-dihydrobenzol - [1,4]-thiazine-3,5-dione-11-O-β-D-glucopyranoside (3), 2-hydroxy-7-hydroxymethyl-8,8-dimethyl-4,8 -dihydrobenzol [1,4]thiazine-3,5-dione-11-O-β-D-glucopyranoside (4), 7-hydroxymethyl-8,8-dimethyl-4,8-dihydrobenzol - [1,4]-thiazine-3,5-dione-(2-O-caffeoyl)-β-D-glucopyranoside (5), xanthialdehyde (6), chrysophanic acid (7), emodin (8), aloe emodin (9), and 7-[(β-D-apiofuranosyl-(1→6)-β-D-glucopyranosyl) oxymethy]-8,8-dimethyl-4,8-dihydrobenzo [1,4]thiazine-3,5-dione (10). The detailed chemical structures are shown in [Table 5] and [Figure 6].
Figure 6: Chemical structure of thiazides reported from Xanthii Fructus

Click here to view
Table 5: Thiazides reported from Xanthii Fructus

Click here to view
{Table 6}


Ahuja and Nigam reported that XF contains anthraquinones. In addition, flavonoids, alkaloids, heterocyclic compounds, adenosine, sterols, fatty acids, coumarins, and other compounds have been identified in XF.[31],[71],[72]

  Analytical Methods Top

Presently, in ChP, the quality of XF is controlled by HPLC and the content of chlorogenic acid is at least 0.25%. Among the abovementioned analytical methods, HPLC is the most frequently used, and is the main method used for the analysis of other compounds.[12] Although HPLC is a simple and accurate method, it does not provide a comprehensive method for the quality control of drugs. Detection of chlorogenic acid content only when determining the content of XF is also a disadvantage because the content of other chemical components in XF has not been determined, which is not favorable for quality control.

Recently, with the development of analytical instruments, there have been many reports on the quality control methods for XF. For phenolic acids and glycosides, commonly used analytical methods include HPLC, high-performance capillary electrophoresis, ultra-performance liquid chromatography (UPLC), ultra-HPLC-triple quadrupole-linear ion trap mass spectrometry (UPLC-QTRAP-MS/MS), and HPLC-photodiode array. For anthraquinones and flavonoids in XF, UPLC-QTRAP-MS/MS is an effective method for the simultaneous determination of these two compounds.[73],[74] Volatile oils can be detected by HPLC-evaporative light-scattering detector and GC-MS. Other methods, such as liquid chromatography-diode array spectrometry/electrospray ionization mass spectrometry (HPLC-DAD/ESI-MS) and UPLC coupled with time-of-fight mass spectrometry (UPLC/Q-TOF-MS), have also been used to determine the chemical content of XF.[40],[68] Furthermore, these methods can detect changes in the active and toxic components of XF with sensitivity before and after processing. In recent years, researchers have studied the relationship between pharmacological activity and the content of chemical constituents in plants. This analytical method may bring about a great change in the quality control of XF.

  Pharmacological Activities and Clinical Application Top

Pharmacological activities

The earliest application of Xanthium in China can be traced back to the Qin dynasty. The roots, stems, leaves, flowers, and fruits of Xanthium have been reported to have medicinal effects in classical works, including XF.[74]

Antimicrobial activity

In vitro, XF has been shown to exert a strong inhibitory effect on many microorganisms, such as Staphylococcus aureus, Pneumococcus, and Group b Streptococcus.[75],[76] It has demonstrated clear bacteriostatic effects on Escherichia coli and Bacillus subtilis, whereby the bacteriostatic effect on E. coli is greater than that on B. subtilis.[77]

Acetone and ethanol extracts of XF have also demonstrated inhibitory effects on Trichoderma rubra. Chloroform extract and n-butanol extract of XF can inhibit Demodex oculi, and mucous toxicity tests found no irritation.[78] The inhibition rate of hepatitis B virus (HBV) DNA by XF polysaccharides was 25%–50%.[63] XF extracts can control the pathological changes caused by HBV without affecting transaminase levels.[79] In addition, an ethanol extract of XF can completely inhibit the growth of herpes virus at 0.1 mg/mL, and has no toxic effect on normal cells.[80]

Anti-inflammatory and analgesic activities

Intraperitoneal injection of methanol extract of XF at 250 mg/kg increased analgesia by 30%–60%. XF has some anti-inflammatory and analgesic effects on writhing reaction induced by subcutaneous injection of 1000 mg/kg acetic acid in mice.[64],[81] The anti-inflammatory mechanism of action has been discussed in recent years. Studies have shown that the product of the MEXS gene is a potent inhibitor of nitric oxide, prostaglandin E2, and tumor necrosis factor-alpha (TNF-α) production. Inducible nitric oxide synthase, cyclooxygenase-2 expression, and TNF-α release were inhibited through the blockade of nuclear factor-kappa B activation by MEXS. XF has an inhibitory effect on the above inflammatory factors to a certain extent.[8],[82]

Anti-allergy effect

Among the various extracts of XF, a 70% alcohol extract was found to have the best anti-allergic effect, and its anti-allergy mechanism may be related to the inhibition of Ca2+ influx in mast cells and the intracellular cyclic adenosine monophosphate (cAMP) content.[83],[84],[85] Dai et al. (in 2008) showed that xanthium can inhibit allergic reactions rapidly. Accordingly, 70% alcohol extracts of XF inhibit anaphylactic shock in mice, passive skin reactions in rats in vivo, and reduce histamine and beta-aminohexase release by rat mast cells in vitro. It was found to have no significant effect on the skin response to histamine or serotonin in rats. These results showed that XF can stabilize hypertrophic cell membranes, reduce the release of histamine and other allergic mediators, and inhibit mast cell-dependent anaphylaxis.[82],[83]

Antioxidant effect

Free radicals have a great influence on oxidation reactions. Extracts of XF have been shown to scavenge superoxide radicals and hydroxyl radicals; they have also been shown to delay and reduce lipid peroxidation.[54] In addition, XF extracts can reduce the lipid peroxide content in tissues and increase the activity of superoxide dismutase. The antioxidant activity of an XF extract was better at scavenging superoxide free radicals, while that of an alcohol extract was better at scavenging hydroxyl free radicals.[86]

Antitumor effect

Using a serum pharmacology method, Wei et al. treated human liver cancer cells cultured in vitro with low, medium, and high doses of XF in mouse serum and 5-fluorouracil, and used clonogenesis and flow cytometry to examine the effects on cell division, proliferation, and apoptosis.[87],[88] The results showed that the XF drug serum had inhibitory and toxic effects on human liver cancer cells. In addition to its effects on human liver cancer cells, XF drug serum displayed clear toxic and inhibitory effects on human brain glioma and S180 sarcoma cells.[4]

Hypoglycemic effect

Monosaccharides are absorbed directly into the bloodstream through the small intestine, whereas disaccharides and polysaccharides are converted into monosaccharides by alpha-glucose liver enzymes.[89] XF can reduce liver glycogen in animals, and a water extract of XF can inhibit the activity of alpha-glucose liver enzymes. The glycosides in XF can significantly reduce the blood glucose level induced by rhamnose, but cannot reduce the hyperglycemia caused by tetrafluorouracil in rats. If rhamnose is injected first, followed by epinephrine, the elevated blood sugar response of epinephrine decreases or is lost. Low, medium, and high doses of an XF decoction can reduce the blood glucose of normal mice, and are able to regulate blood glucose and maintain blood glucose stability. The medium- and high-dose XF decoctions can significantly reduce the blood glucose of hyperglycemia mice and improve the glucose tolerance of hyperglycemia mice.[66],[90],[91]

Effects on immune function

Animal experiments have shown that XF has an inhibitory effect on both humoral and cellular immunity, with a greater effect on cellular immunity. In addition, XF displayed an inhibitory effect on the immune response of mononuclear macrophages.[92] XF is not conducive to the increase in capillary permeability caused by histamine. XF significantly inhibits the expression of the interleukin-2 receptor, regulates the immune imbalance of Th cells in patients with asthma, and inhibits the release of inflammatory transmitters.[56] Experiments have shown that XF induces different degrees of weight gain in immune organs. Specifically, the weight of the thymus and spleen increased with an increasing dose, and the serum hemolysin value increased in mice.[93]

Effects on other physiological systems

In vitro animal experiments have shown that XF has an inhibitory effect on the heart, and can slow the heart rate and reduce myocardial systolic function. Animal models have revealed different effects of XF on blood vessels, which mainly functions to dilate blood vessels.[94] In addition, XF extracts exerted an obvious antithrombin effect. Methanol extract had good effect on the recovery of cholesterol triglyceride level, enhanced the phospholipid level, and inhibited the cholesterol and triglyceride level.[95] Moreover, the results of the study by Duan Xiaomao (2006) showed that compared with normal saline, chemical stimulation with XF and alcohol prolonged the latent period of cough in mice, and significantly decreased the number of coughs. Compared with codeine, there was little difference in the antitussive effect. This indicates that XF exerts an antitussive effect.

Clinical application

XF is used to clinically treat rhinitis, sinusitis, urinary tract infection, upper respiratory infection, hypoglycemia, intractable toothache, malaria, chronic bronchitis, mumps, allergic diseases, urticaria, tumor, dermatitis, diarrhea, and otitis media.[90],[91],[92],[93],[94],[95],[96],[97],[98]

  Toxicity and Side Effects Top

The symptoms of XF poisoning are generally the same, regardless of the drug administration route. The activity of laboratory animals decreased upon XF administration.[95] Compared to normal animals, experimental animals were less active and responsive to external stimuli and had irregular breathing and extreme breathing difficulties with paroxysmal convulsion before they died.[96] The absolute lethal dose (LD100) of a 25% XF emulsion intraperitoneally injected into rabbits was 10 mL/kg, and the median lethal dose (LD50) in mice was 7.5 mL/kg.[97] Histopathological examination of the main organs of various animals after poisoning revealed the same lesions, differing only in the degree of severity. Among them, liver injury was the most serious.[98] The livers of dead animals presented degenerative disease or even necrosis, and epithelial turbiditis in the curved tube of the kidney was similar to the symptoms of carbon tetrachloride poisoning.[99] Therefore, the main consequence of XF poisoning is liver necrosis; death may be due to convulsion caused by secondary brain edema. Many studies have found that the toxic components of XF may be related to toxic proteins or xanthium glycosides and alkaloids.[100] The toxicity of XF extract is proportional to the drug concentration.

The toxic components of XF were determined for medicinal xanthium herbs, decoction pieces, and representative formulations containing xanthium (Biyuanshu oral liquid). The results showed that toxicity was observed as follows: decoction pieces > medicinal material > prescription preparation. The results of acute toxicity tests showed that all the three preparations in mice resulted in toxicity as follows: decoction pieces > medicinal material > prescription preparation. The toxicity of decoction pieces was greater than that of medicinal herbs, which was consistent with the trend observed for atractylode content.[99]

Clinical adverse reactions of XF have been reported in clinical studies and mainly manifest in terms of damage to the skin, digestive system, nervous system, and cardiovascular system. The specific performance was as follows:[94],[96],[97],[98],[99],[100],[101] first, skin lesions manifest as a systemic rash or contact dermatitis; second, digestive system toxicity is characterized by nausea, vomiting, loss of appetite, abdominal distention, diarrhea, hematochezia, liver and kidney pain, liver enlargement, ascites, liver coma, abnormal liver function, and liver failure; third, nervous system symptoms include dizziness, headache, restlessness, convulsions, drowsiness, confusion, coma, cerebral edema, and aphasia; fourth, damage to the cardiovascular system includes chest tightness, shortness of breath, decreased blood pressure, arrhythmia, and atrioventricular block; fifth, urinary system toxicity is characterized by edema, oliguria, urinary closure, hematuria, urinary incontinence, renal dysfunction, and acute renal failure; sixth, adverse effects of XF on the respiratory system include dyspnea, irregular respiratory rhythms, and pulmonary edema; and seventh, damage to the hematopoietic system involves thrombocytopenic purpura, gingival bleeding, and cutaneous mucosal bleeding. Other side effects include neuroedema, nosebleed, lip swelling, hoarseness, and difficulty swallowing.

  Processing and Reducing Toxicity Top

XF is toxic to many organs, especially the liver and kidney.[66],[101] Because processing can reduce the toxicity of XF, clinical use generally involves processed products of XF.[23] In the Liu and Song dynasties of the southern and northern dynasties of China, XF was steamed with Polygonatum sibiricum (Thunder Lord). The Tang Dynasty employed a method of burning ashes. In the Song Dynasty of China, methods such as burning ashes, stir-frying (Shenghuifang), stir-frying incense to stab (certificate type), and baking (First aid) are recorded. In the Ming Dynasty of China, stir-fry and steaming were commonly used, such as pastry (Puji formula), micro stir-fry (medicine), steaming with P. sibiricum juice (introduction), single steaming (Dafa), stir-fry to remove thorns, and wine mix steaming

(Chengya). The Qing Dynasty of China used methods such as stir-frying and smashing (the law), and stir-frying, stir-frying with wine (Materia Medica). Presently, XF processing technology is generally divided into net and frying processes.[99] The cleaning process refers to the removal of cockle thorns and impurities. Common methods for the frying process include the clear frying method and the blanching method.[102] During this process, the blanching method is generally selected.[103],[104],[105],[106] The optimal way to prepare XF to reduce its toxicity is to fry it and then grind the spines. For clinical use, it must be fried until brown to reduce toxicity.[107]

The chemical composition and pharmacological action of XF also change substantially before and after processing.

For phenolic acid, the content of total phenolic acid in XF was significantly different with different processing temperatures and times, and the content ranged from 7.99 to 9.69 mg/g. The contents of new chlorogenic acid, chlorogenic acid, 1,5-dicaffeinic acid, and total phenolic acid in the decoction of XF after frying increased with increasing preparation temperature.[108],[109]

For volatile and fatty oils, the content of fatty oil was significantly higher than that of crude oil, but the physical constant changed little, and the specific gravity and acid values decreased slightly upon processing. Woody et al. (2013) qualitatively analyzed the changes in volatile oil and fat oil before and after processing of XF by GC-MS. The volatile oil in the raw products of XF included 18 chemical constituents, and the volatile oil in the fried products of XF included 13 chemical constituents. Four components of XF were identified in raw and fried fat oil. The results showed that the chemical composition of volatile oil was different before and after processing, while the composition of fat oil was not changed.[110]

For water-soluble glycosides and mixed protein, Han (2014) found that the rate of XF seed protein extraction had significantly reduced after frying.[111] Duorui et al. (2013) found that the content of hydroxy atractylodes decreased to 10% after XF was fried to yellow. During the frying process, hydroxy atractylodes were converted to atractylodes, and no carboxyl atractylodes were present after XF was fried to char. Moreover, atractylodes increased at temperatures of 140°C–260°C and decreased at temperatures above 260°C.

In 2008, Chen Daihong showed that processing could increase the analgesic effect and reduce drug toxicity.[43] In 2012, Wu Hui compared differences in the toxicity of toxic parts before and after frying by means of an acute toxicity pharmacological test in mice, and showed that the fried products of xanthium have reduced toxicity.[56] Zhao et al. showed that a water decoction and fatty oil emulsion of XF were more effective than the raw products against S. aureus and Pneumococcus.[112] In 2016, the anti-inflammatory effects of raw XF seed products were found to exceed those of fried products, and the effects of processed drugs on blood glucose lowering were better than those of raw products.[43] There was no significant difference between raw and fried xanthium in terms of blood glucose lowering.[113]

XF presents reduced toxicity after stir-frying. Therefore, XF should be used for medicinal purposes after stir-frying, but the temperature should be controlled strictly to prevent the loss of efficacy.

  Future Perspectives and Conclusions Top

XF has been used for years in China as a drug to treat sinusitis. To date, national and international scientists have isolated 100 chemical components from XF. The pharmacological activities of its chemical components have mainly focused on phenylpropanoids and sesquiterpenoids, while studies on lignanoids and thiazides are relatively rare. In addition, the decreased toxicity of XF before and after processing may be related to changes in the chemical composition; however, experimental evidence to support this is lacking. In this article, the chemical components of XF are summarized, which is expected to be helpful for the research and development of new drugs, especially in terms of its pharmacodynamics and structure–activity relationship.

In terms of pharmacology, XF is often used in the clinical treatment of allergic diseases, suggesting that it may have immunosuppressive and anti-allergy effects; however, there has been no reasonable explanation to explain its clinical efficacy. Network pharmacology is commonly used to analyze the mechanism of action of XF, and may represent a suitable method for studies on its mechanism of action. At the same time, researchers should perform further in vitro and in vivo experiments to elucidate the full therapeutic potential of XF.

With the development of natural medicinal chemistry, more attention must be paid to TCM. XF contains abundant medicinal plant resources and is widely distributed in China. In this article, the chemical components, analytical methods, pharmacological effects, clinical application, toxicity, and processing technology of XF are systematically reviewed, and some issues that need to be overcome are discussed, in order to provide a direction for the future study of XF.


This project was financially supported by the National Natural Science Foundation of China (No. 81703684, 81803690, and 81973604); the Graduate Innovative Research Project Foundation of Heilongjiang University of Chinese Medicine (No. 2019yjsc × 013); the Innovative Talents Funding of Heilongjiang University of Chinese Medicine (No. 2018RCD25); the National Natural Science Foundation Matching Project (No. 2018PT02); the Postdoctoral Initial Fund of Heilongjiang Province, the University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province (No. UNPYSCT 2017219 and UNPYSCT 2017215); the National Natural Science Foundation Matching Project (No. 2017PT01); Heilongjiang Postdoctoral Scientific Research Developmental Fund (No. LBH Q16210 and LBH-Q17161); the Natural Science Foundation of Heilongjiang Province (No. H2015037); the Heilongjiang University of Chinese Medicine Doctoral Innovation Foundation (No. 2014bs05); and the Application Technology Research and Development Projects of Harbin Technology Bureau (No. 2014RFQXJ149).

Availability of data and materials

All reported or analyzed data in this review were extracted from published articles.

Financial support and sponsorship


Conflicts of interest

There are no conflicts of interest.

  References Top

Rui D, Jin-Hai YI, Yu-Hong L, Yun-Hua L, Yan C, Zhi-Fang H. Effects on six ingredients in Xanthii Fructus with different parching processing. West China J Pharm Sci 2015;30:697-700.  Back to cited text no. 1
Hsu FL, Chen YC, Cheng JT. Caffeic acid as active principle from the fruit of Xanthium strumarium to lower plasma glucose in diabetic rats. Planta Med 2000;66:228-30.  Back to cited text no. 2
Han T, Li HL, Zhang QY, Han P, Zheng HC, Rahman K, et al. Bioactivity-guided fractionation for anti-inflammatory and analgesic properties and constituents of Xanthium strumarium L. Phytomedicine 2007;14:825-9.  Back to cited text no. 3
Piloto-Ferrer J, Sánchez-Lamar Á, Francisco M, González ML, Merino N, Aparicio G, et al. Xanthium strumarium´s xanthatins induces mitotic arrest and apoptosis in CT26WT colon carcinoma cells. Phytomedicine 2019;57:236-44.  Back to cited text no. 4
Zhang XM, Zhang ZH. The study of intoxication and toxicity of Fructus Xanthii. Zhong Xi Yi Jie He Xue Bao 2003;1:71-3.  Back to cited text no. 5
Jiang H, Yang L, Liu C, Hou H, Wang Q, Wang Z, et al. Four new glycosides from the fruit of Xanthium sibiricum Patr. Molecules 2013;18:12464-73.  Back to cited text no. 6
Hai J, Wenjing M, Liu Y, Xudong X, Meiling Y, Xinyue G. Study on the Chemical Constituents of Caffeoylquinic Acid in Xanthii Fructus. J Changchun Univ Chin Med 2019;35:110-3.  Back to cited text no. 7
Dai YH, Cui Z, Li JL, Wang D. A new thiaziedione from the fruits of Xanthium sibiricum. J Asian Nat Prod Res 2008;10:343-7.  Back to cited text no. 8
Jiang H, Yang L, Xing XD, Yan ML, Guo XY, Su XL, et al. Study on lignans from Xanthii Fructus. Zhongguo Zhong Yao Za Zhi 2018;43:2097-103.  Back to cited text no. 9
Huang WH, Yu JG, Sun L, Guo BL. Study on the chemical constituents of Chinese traditional medicine Xanthium sibiricum. J Tradit Chin Med 2005;30:1027-8.  Back to cited text no. 10
Han T, Zhang QY, Zhang H, Wen J, Wang Y, Huang BK, et al. Authentication and quantitative analysis on the chemical profile of Xanthium fruit (Cang-Er-Zi) by high-performance liquid chromatography-diode-array detection tandem mass spectrometry method. Anal Chim Acta 2009;634:272-8.  Back to cited text no. 11
Duo R, Chen Y, Liu Y, Huang Z, Liu Y, Yi J. Determination of carboxyatractyloside and atractyloside in Xanthii Fructus by HPLC. Zhongguo Zhong Yao Za Zhi 2012;37:2313-6.  Back to cited text no. 12
Geng ZL, Wei HY, Li XJ, Liu BM. GC-MS analysis of chemical constituents of volatile oil from xanthium. J Tradit Chin Med Phar 2006;13:248-250.  Back to cited text no. 13
Taruscio TG, Barney DL, Exon J. Content and profile of flavanoid and phenolic acid compounds in conjunction with the antioxidant capacity for a variety of northwest Vaccinium berries. J Agric Food Chem 2004;52:3169-76.  Back to cited text no. 14
Yingxiang L, Zhizhong J, Ying X, Shengchang T, Baofeng Z. Synthesis and antiinflammatory activity of derivatives of 4 cinnamoylferulic acid phenolic Ester. Chin J Med Chem 1997;1:18-22.  Back to cited text no. 15
Proestos C, Chorianopoulos N, Nychas GJ, Komaitis M. RP-HPLC analysis of the phenolic compounds of plant extracts. investigation of their antioxidant capacity and antimicrobial activity. J Agric Food Chem 2005;53:1190-5.  Back to cited text no. 16
Sarikaya SB, Gülçin I, Supuran CT. Carbonic anhydrase inhibitors: Inhibition of human erythrocyte isozymes I and II with a series of phenolic acids. Chem Biol Drug Des 2010;75:515-20.  Back to cited text no. 17
Hartmann F, Ruge W. Studies on the phenolic acid pattern in liver diseases. Dtsch Arch Klin Med 1962;208:298-322.  Back to cited text no. 18
Rabiner SF, Molinas F. The role of phenol and phenolic acids on the thrombocytopathy and defective platelet aggregation of patients with renal failure. Am J Med 1970;49:346-51.  Back to cited text no. 19
Zhuang Y, Qin K, Liu X, Cai B, Cai H. Ultra-high-performance liquid chromatography with tandem mass spectrometry method for determination of four compounds in rat plasma after oral administration of Xanthii fructus and stir-fried Xanthii Fructus extracts. Biomed Chromatogr 2019;33:e4464.  Back to cited text no. 20
Tian J, Xia YF, Fang KH. Simultaneous determination of eight phenolic acids in Xanthium sibiricum by HPLC. Chin Tradit Pat Med 2013;36:1623-6.  Back to cited text no. 21
Han T, Li H, Zhang Q, Zheng H, Qin L. New thiazinediones and other components from Xanthium strumarium. Chem Nat Compd+ 2006;42:567-70.  Back to cited text no. 22
Shi YS, Li L, Liu YB, Ma SG, Li Y, Qu J, et al. A new thiophene and two new monoterpenoids from Xanthium sibiricum. J Asian Nat Prod Res 2015;17:1039-47.  Back to cited text no. 23
Li N, Zhang WZ. Studies on chemical constituents of xanthium sibiricum patrin ex widder. J Qiqihar Univ 2016;32:51.  Back to cited text no. 24
Agata I, Goto S, Hatano T, Nishibe S, Okuda T. 13,5-tri-o-caffeoylquinic acid from xanthium strumarium. Phytochemistry 1993;33:508-9.  Back to cited text no. 25
Chen J, Wang R, Shi YP. Chemical constituents from Xanthii Fructus. Chinese Tradit Herbal Drugs 2013;44:1717-20.  Back to cited text no. 26
Hwang S, Wang Z, Yoon H, Lim SS. Xanthium strumarium as an inhibitor of α-glucosidase, protein tyrosine phosphatase 1β, protein glycation and ABTS+for diabetic and its complication. Molecules 2016;21:1241.  Back to cited text no. 27
Han T, Li HL, Hu Y, Zhang QY, Huang BK, Zheng HC, et al. Phenolic acids in Fructus Xanthii and determination of contents of total phenolic acids in different species and populations of Xanthium in China. J Chin Integr Med 2006;4:194-8.  Back to cited text no. 28
Yuan HE. Study on the Chemical Constituents of Herba Commelinae and Fructus Xanthii. Master's Thesis, Jinan University, Guangdong, China; 2014.  Back to cited text no. 29
Su T, Cheng BC, Fu XQ, Li T, Guo H, Cao HH, et al. Comparison of the toxicities, bioactivities and chemical profiles of raw and processed Xanthii Fructus. BMC Complem Altern M 2015;16:24.  Back to cited text no. 30
Kan S, Chen G, Han C, Chen Z, Song X, Ren M, et al. Chemical constituents from the roots of Xanthium sibiricum. Nat Prod Res 2011;25:1243-9.  Back to cited text no. 31
Yu-Ling Q, Ying-Hui D, Dong W, Zheng C. Chemical constituents in the fruits of Xanthium sibiricum. Chin J Med Chem 2010;20:214-6+225.  Back to cited text no. 32
Jiang H, Yang L, Ma GX, Xing XD, Yan ML, Zhang YY, et al. New phenylpropanoid derivatives from the fruits of Xanthium sibiricum and their anti-inflammatory activity. Fitoterapia 2017;117:11-5.  Back to cited text no. 33
Kawai Y, Kumagai H, Kurihara H, Yamazaki K, Sawano R, Inoue N. β-glucosidase inhibitory activities of phenylpropanoid glycosides, vanicoside a and b from polygonum sachalinense rhizome. Fitoterapia 2006;77:456-459.  Back to cited text no. 34
Dobler S, Petschenka G, Pankoke H. Coping with toxic plant compounds-the insect's perspective on iridoid glycosides and cardenolides. Phytochemistry 2011;72:1593-604.  Back to cited text no. 35
Nikles S, Heuberger H, Hilsdorf E, Schmücker R, Bauer R. Influence of processing on the content of toxic carboxyatractyloside and atractyloside and the microbiological status of xanthium sibiricum fruits (Cang'erzi). Planta Med 2015;81:1213-20.  Back to cited text no. 36
Yin RH, Bai X, Feng T, Dong ZJ, Li ZH, Liu JK. Two new compounds from Xanthium strumarium. J Asian Nat Prod Res 2016;18:354-9.  Back to cited text no. 37
Craig JC, Mole ML, Billets S, El-Feraly F. Isolation and identification of the hypoglycemic agent, carboxyatracrylate, from Xanthium strumarium. Phytochemistry 1976;15:1178-80.  Back to cited text no. 38
Hai J, Liu Y, Xu-Dong X, Yan-Yan Z, Mei-Ling Y, Bing-You Y. Chemical constituents from fruits of Uanthium Sibiricum. Chin Tradit Herbal Drugs 2017;48:47-51.  Back to cited text no. 39
Cheng Z, Wang L, Chen B, Li F, Wang M. Chemical constituents from Fructus xanthii. Chin J Appl Environ Biol 2011;17:350-2.  Back to cited text no. 40
Yoon HN, Lee MY, Kim JK, Suh HW, Lim SS. Aldose reductase inhibitory compounds from Xanthium strumarium. Arch Pharm Res 2013;36:1090-5.  Back to cited text no. 41
Yu J, Song MZ, Wang J, Li YF, Lin P, Que L, et al. In vitrocytotoxicity and in vivo acute and chronic toxicity of Xanthii Fructus and its processed product. Biomed Res Int 2013;2013:403491.  Back to cited text no. 42
Jiang H, Zhang YY, Zhang Y, Yang L, Wang QH, Kuang HX. Isolation and identification of chemical constituents from the fruit of Patr. Inf Tradit Chin Med 2016;33:8-10.  Back to cited text no. 43
Huang WH, Yu JG, Sun L, Guo BL, Li DY. Studies on chemical constituents of Xanthium sibiricum. Chin J Chin Mater Med 2005;30:1027-8.  Back to cited text no. 44
Shi YS, Liu YB, Ma SG, Li Y, Qu J, Li L, et al. Bioactive Sesquiterpenes and Lignans from the Fruits of Xanthium sibiricum. J Nat Prod 2015;78:1526-35.  Back to cited text no. 45
Bui VB, Liu ST, Zhu JJ, Xiong J, Zhao Y, Yang GX, et al. Sesquiterpene lactones from the aerial parts of Xanthium sibiricum and their cytotoxic effects on human cancer cell lines. Phytochem Lett 2012;5:685-9.  Back to cited text no. 46
Zhang D, Ge H, Zou J, Tao X, Chen R, Dai J. Periconianone A, a New 6/6/6 carbocyclic sesquiterpenoid from endophytic fungus periconia sp. with Neural Antiinflammatory Activity. ChemInform 2014;45:1410-3.  Back to cited text no. 47
Kirana C, McIntosh GH, Record IR, Jones GP. Antitumor activity of extract of Zingiber aromaticum and its bioactive sesquiterpenoid zerumbone. Nutr Cancer 2003;45:218-25.  Back to cited text no. 48
Nguyen TT, Nguyen TT, Lee H, Lee B, Min BS, Kim JA. Anti-allergic and Cytotoxic Effects of Sesquiterpenoids and Phenylpropanoids Isolated from Magnolia biondii. Nat Prod Commun 2017;12:1934578X1701201005.  Back to cited text no. 49
Rabe T, van Staden J. Isolation of an antibacterial sesquiterpenoid from Warburgia salutaris. J Ethnopharmacol 2000;73:171-4.  Back to cited text no. 50
Yamahara J, Li YH, Tamai Y. Anti-ulcer effect in rats of bitter cardamon constituents. Chem Pharm Bull (Tokyo) 1990;38:3053-4.  Back to cited text no. 51
Levine RB, Fahrbach SE, Weeks JC. Steroid hormones and the reorganization of the nervous system during metamorphosis. Seminars in Neurosci 1991;3:437-47.  Back to cited text no. 52
McMillan C, Chavez PI, Mabry TJ. Sesquiterpene lactones of Xanthium strumarium in a Texas population and in experimental hybrids. Biochem Syst Ecol 1975;3:137-141.  Back to cited text no. 53
Li WD, Wu Y, Zhang L, Yan LG, Yin FZ, Ruan JS, et al. Characterization of xanthatin: Anticancer properties and mechanisms of inhibited murine melanoma in vitro and in vivo. Phytomedicine 2013;20:865-73.  Back to cited text no. 54
Han T, Zhang H, Li HL, Zhang QY, Zheng HC, Qin LP. Composition of supercritical fluid extracts of some Xanthium species from China. Chem Nat CompD+2008;44:814-6.  Back to cited text no. 55
Karmakar UK, Ishikawa N, Toume K, Arai MA, Sadhu SK, Ahmed F, et al. Sesquiterpenes with TRAIL-resistance overcoming activity from Xanthium strumarium. Bioorgan Med Chem 2015;23:4746-54.  Back to cited text no. 56
Wang L, Wang J, Li F, Liu X, Chen B, Tang YX, et al. Cytotoxic sesquiterpene lactones from aerial parts of Xanthium sibiricum. Planta Med 2013;79:661-5.  Back to cited text no. 57
Malik MS, Sangwan NK, Dhindsa KS. Xanthanolides from Xanthium strumarium. Phytochemistry 1993;32:206-7.  Back to cited text no. 58
Qin L, Han T, Li H, Zhang Q, Zheng H. A new thiazinedione from Xanthium strumarium. Fitoterapia 2006;77:245-6.  Back to cited text no. 59
Mahmoud AA. Xanthanolides and xanthane epoxide derivatives from Xanthium strumarium. Planta Med 1998;64:724-7.  Back to cited text no. 60
Hu DY, Yang SY, Yuan CS, Han GT, Shen HM. Isolation and identification of chemical constituents in Xanthium sibiricum. Chin Tradit Herbal Drugs 2012;43:640-4.  Back to cited text no. 61
Jiang H, Yang L, Xing XD, Yan ML, Guo XY, Su XL, et al. Chemical constituents of terpenoids from Xanthium strumarium. Chin Tradit Pat Med 2018;40:2461-6.  Back to cited text no. 62
Cutler HG, Cole RJ. Carboxyatractyloside: A Compound from Xanthium strumarium and Atractylis gummifera with Plant Growth Inhibiting Properties. The Probable” Inhibitor A”. J Nat Prod 1983;46:609-13.  Back to cited text no. 63
Huang MH, Wang BS, Chiu CS, Amagaya S, Hsieh WT, Huang SS, et al. Antioxidant, antinociceptive, and anti-inflammatory activities of Xanthii Fructus extract. J Ethnopharmacol 2011;135:545-52.  Back to cited text no. 64
Hong SH, Jeong HJ, Kim HM. Inhibitory effects of Xanthii fructus extract on mast cell-mediated allergic reaction in murine model. J Ethnopharmacol 2003;88:229-34.  Back to cited text no. 65
Ingawale AS, Sadiq MB, Nguyen LT, Ngan TB. Optimization of Extraction Conditions and Assessment of Antioxidant, α-Glucosidase Inhibitory and Antimicrobial Activities of Xanthium strumarium L. fruits. Biocatal Agricultural Biotechnol 2018;14:40-7.  Back to cited text no. 66
Jiang H, Yang L, Xing XD, Yan ML, Guo XY, Su XL, et al. Studies on the chemical constituents of lignans in Xanthium sibiricum. Chin J Tra Chin Med 2018;43:2097-103.  Back to cited text no. 67
Juan-Xiu L, Yi-Yuan L, Xun-Hong L, Jian-Ping S, Ya H, Yang MA. Simultaneous determination of fourteen fatty acids in Xanthii Fructus by derivatized GC-MS. Natl Product Res Develop 2016;28:76-82.  Back to cited text no. 68
Ma YT, Huang MC, Hsu FL, Chang HF. Thiazinedione from xanthium strumarium. Phytochemistry (Oxford) 1998;48:1083-5.  Back to cited text no. 69
Lee CL, Huang PC, Hsieh PW, Hwang TL, Hou YY, Chang FR, et al. Xanthienopyran, a new inhibitor of superoxide anion generation by activated neutrophils, and further constituents of the seeds of\\r, xanthium strumarium. Planta Med 2008;74:1276-9.  Back to cited text no. 70
Ahuja MM, Nigam SS. Chemical examination of the essential oil from the leaves of Xanthium strumarium (Linn.). Flavour Industry 1970;1:627-30.  Back to cited text no. 71
Juanxiu L, Yiyuan L, Xunhong L, Jianping S, Yujiao H, Shengnan W. Simultaneous determination of phenolic acids, anthraquinones and flavonoids in Xanthii Herba and Xanthii Fructus by uplc-qtrap-ms/ms%uplc-qtrap-ms/ms. J Chin Mass Spectrometry Soc 2016;037:542-553.  Back to cited text no. 72
Chen F, Hao F, Li C, Gou J, Lu D, Gong F, et al. Identifying three ecological chemotypes of Xanthium strumarium glandular trichomes using a combined NMR and LC-MS method. PLoS One 2013;8:e76621.  Back to cited text no. 73
Jiang H, Yang L, Xing X, Yan M, Guo X, Yang B, et al. Chemometrics coupled with UPLC-MS/MS for simultaneous analysis of markers in the raw and processed Fructus Xanthii, and application to optimization of processing method by BBD design. Phytomedicine 2019;57:191-202.  Back to cited text no. 74
Liu Y, Wu ZM, Lan P. Experimental study on effect of Fructus Xanthii extract on duck hepatitis B virus. Lishizhen Med Mater Med Res 2009;20:1776-7.  Back to cited text no. 75
Xu DL, Wang R. The effective mite-killing portion of Fructus Xanthii. Chinese J Pharm Analysis 2010;30:2048-51.  Back to cited text no. 76
Zhuang YS, Hu J, Cai H, Qin KM, Yang B, Liu X, et al. Advanced study on chemical constituents and pharmaceutical activities of Xanthium strumarium. J Nanjing Univ Tradit Chin Med 2017;33:428-32.  Back to cited text no. 77
Su JQ, Zhao YC, Chang YL, Zhang X, Liu CY, Chen GY, et al. Optimization of extraction process and antibacterial activity of flavonoids in Xanthium fruticum. Feed Res 2017;3:28-31.  Back to cited text no. 78
Yeom M, Kim JH, Min JH, Hwang MK, Jung HS, Sohn Y. Xanthii fructus inhibits inflammatory responses in LPS-stimulated RAW 264.7 macrophages through suppressing NF-κB and JNK/p38 MAPK. J Ethnopharmacol 2015;176:394-401.  Back to cited text no. 79
Qu J, Deng S, Li L, Liu Y, Li Y, Ma S, et al. Cytotoxic dimeric xanthanolides from fruits of Xanthium Chinense. Phytochemistry 2016;132:115-22.  Back to cited text no. 80
Kim IT, Park YM, Won JH, Jung HJ, Park HJ, Choi JW, et al. Methanol extract of Xanthium strumarium L. possesses anti-inflammatory and anti-nociceptive activities. Biol Pharm Bull 2005;28:94-100.  Back to cited text no. 81
Yan GH, Jin GY, Li GS, Cui CA, Quan GH, Jin DS, et al. The possible mechanism of inhibitory effect of Xanthium strumarium on mast cells activated by compound 48/80. Progress Anatomical Sci 2010;16:164-6.  Back to cited text no. 82
Peng W, Ming QL, Han P, Zhang QY, Jiang YP, Zheng CJ. Anti-allergic rhinitis effect of caffeoylxanthiazonoside isolated from fruits of Xanthium strumarium L. In rodent animals. Phytomedicine 2014;21:824-9.  Back to cited text no. 83
Kang DG, Yun CK, Lee HS. Screening and comparison of antioxidant activity of solvent extracts of herbal medicines used in Korea. J Ethnopharmacol 2003;87:231-6.  Back to cited text no. 84
Su XG, Huang TL, Wang NS. Antioxidant compounds and radical scavenging property of Fructus Xanthii. Tradit Chin Drug Res Clin Pharmacol 2007;18:47-9.  Back to cited text no. 85
Takeda S, Matsuo K, Yaji K, Okajima-Miyazaki S, Harada M, Miyoshi H. (−)-Xanthatin selectively induces GADD45γ and stimulates caspase-independent cell death in human breast cancer MDA-MB-231 cells. Chem Res Toxicol 2011;24:855-65.  Back to cited text no. 86
Zhang L, Ruan J, Yan L, Li W, Wu Y, Tao L. Xanthatin induces cell cycle arrest at G2/M checkpoint and apoptosis via disrupting NF-κB pathway in A549 non-small-cell lung cancer cells. Molecules 2012;17:3736-50.  Back to cited text no. 87
Wei AQ, Li XW, Lian XZ, Yu FR. Inhibitory effect of xanthium on human liver cancer cell proliferation. J Ecolog Sci 2011;30:647-9.  Back to cited text no. 88
Zhang M, Wu Y, Mu CH, Li QX. Study on the Effect of Xanthium on Blood Glucose in Mice. Lishizhen Med Mater Med Res 2009;20:669-71.  Back to cited text no. 89
Yang L, Chen L, Xu S, Zeng X, Feng Y, Xie P. RRLC-MS/MS method for the quantitation of atractyloside in Fructus Xanthii (Xanthium sibiricum). Anal Methods-UK 2013;5:2093-7.  Back to cited text no. 90
Li JF. Effects of Xanthium and Xinyi on Th1/Th2 ratio and inflammatorytransmitters in patients with bronchial asthma. J Integ Tradit Chin West Med 2012;21:1057- 8.  Back to cited text no. 91
Pan JH, Wang YL, Xie MR, Yu FR. Effect of xanthium extract on tumor growth and immune function of S180 tumour-bearing mice. Chin Clin Study 2013;26:317-9.  Back to cited text no. 92
Zhuang Y, Qin K, Yu B, Liu X, Cai B, Cai H. A metabolomics research based on UHPLC-ESI-Q-TOF-MS coupled with metabolic pathway analysis: Treatment effects of stir-frying Xanthii Fructus on allergic rhinitis in mice model. Biomed Chromatogr 2018;32:e4352.  Back to cited text no. 93
Hu XX, An J, Wang GZ. Effects of different processing temperatures on the contents of main active components and toxic components in Fructus xanthii. Hunan J Tradit Chin Med 2015;31:1.  Back to cited text no. 94
Jiang H, Yang L, Xing X, Yan M, Guo X, Hou A. A UPLC-MS/MS application for comparisons of the hepatotoxicity of raw and processed Xanthii Fructus by energy metabolites. RSC Adv 2019;9:2756-62.  Back to cited text no. 95
Yan LC, Zhang TT, Wu Y, Zhao JN, Song J, Hua H. Study on the toxic effects of cangerum and atractyloside on primary hepatocytes in rats. Pharm Clin Tradit Chin Med 2012;3:36-9.  Back to cited text no. 96
Chuan-Meng L, Hai-Peng C, Liu-Ping T, Ke Y, Chun-Hui Z. Pharmacological effect and toxicity of Xanthii Fructus. Chin J Experim Tradit Med Form 2019;9:207-13.  Back to cited text no. 97
Rong B, Yan-Wei G. Determination of the content of altractyloside in fructus xanthii. Henan Tradit Chin Med 2015;35:2267-9.  Back to cited text no. 98
Fu B, Guo HH, Deng H, Xiao AJ. Determination of total atractyloside in the toxic component of fructus xanthium. Chin J Exper For 2013;19:124-5.  Back to cited text no. 99
Han YQ, Hong Y, Sun YH, Li GD, Wang YZ. Research progress on processing technology and quality control methods of xanthium. J Jiangxi Univ Tradit Chin Med 2013;25:87-90.  Back to cited text no. 100
Jiang H, Yang L, Xing X, Yan M, Guo X, Yang B, et al. HPLC-PDA combined with chemometrics for quantitation of active components and quality assessment of raw and processed fruits of Xanthium strumarium L. Molecules 2018;23:243.  Back to cited text no. 101
An J, Wang YD, Sheng CC, Wang GZ. Comparative analysis of carboxyatractyloside and atractyloside contents in Xanthii Fructus before and after processing. Chin J Pharm Analysis 2013;33:1910-3.  Back to cited text no. 102
Liu JJ, Chen SL, Wang LY. Historical evolution and modern research of xanthium processing. Lishizhen Med Mater Med Res 2000;11.  Back to cited text no. 103
Du R, Zhang B, Fu QY. Content comparison of chlorogenic acid and 1, 5-dicaffeinic acid before and after processing of xanthium fruticum. Chin J Hos Pharm 2014;34:1576-8.  Back to cited text no. 104
Zhao H, Cai H, Liu J X, Wang SN, Liu XH, Yan Y, et al. Simultaneous determination of phenolic acids, anthraquinones, and flavonoids in the aerial part and the fruit of xanthium sibiricum by LC-ESI-QTRAP-MS/MS. Curr Pharm Anal 2019;15:542-53.  Back to cited text no. 105
Sheng CC, An J, Nie L, Wang GZ. Comparison of total phenolic acid content of xanthium before and after stir-frying. J Hubei Univ Chin Med 2013;15:36-8.  Back to cited text no. 106
Rong DU, Guang-Bo Z, Qiao-Yan FU, Pharmacy DO. Comparative analysis of chlorogenic acid and 1,5-dicaffeoylquinic acid contents in fructus xanthii before and after processing. Chin J Hospital Pharm 2014;34:1576-8.  Back to cited text no. 107
Changcui S, Jing AN, Lei N, Guangzhong W. Xanthium total phenolic content before and after frying comparison. J Hubei Univ Chin Med 2013;15:36-8.  Back to cited text no. 108
Rui D, Jin-Hai YI, Yu-Hong L, Yun-Hua L, Yan C, Zhi-Fang H. Effects on six ingredients in Xanthii fructus with different parching processing. West Chin J Pharm Sci 2015;30:697-700.  Back to cited text no. 109
Di HU, Yaodeng W, Hui WU, Jing AN, Guangzhong W. Analysis of essential oil and fatty oil from Xanthii Fructus before and after Stir-frying by GC-MS. J Hubei Univ Chin Med 2012;14:6.  Back to cited text no. 110
Han YQ, Hong Y, Xia LZ, Gao JR, Wang YZ, Sun YH, et al. Optimization of processing technology for xanthii fructus by UPLC fingerprint technique and contents of toxicity ingredient. Zhongguo Zhong Yao Za Zhi 2014;39:1248-54.  Back to cited text no. 111
Zhao CS. Effects of different processing methods on composition and efficacy of Xanthium. Lishizhen Med Mater Med Res 2002;9:522.  Back to cited text no. 112
Jin CS, Wu DL, Zhang JS. Effects of different processing method on constituents and pharmacological action of fructus xanthi. J Anhui Univ Chin Med 2000;1:54-6.  Back to cited text no. 113


  [Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5], [Figure 6]

  [Table 1], [Table 2], [Table 3], [Table 4], [Table 5]


    Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
    Access Statistics
    Email Alert *
    Add to My List *
* Registration required (free)  

  In this article
Analytical Methods
Pharmacological ...
Toxicity and Sid...
Processing and R...
Future Perspecti...
Article Figures
Article Tables

 Article Access Statistics
    PDF Downloaded31    
    Comments [Add]    

Recommend this journal