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ORIGINAL ARTICLE
Year : 2021  |  Volume : 7  |  Issue : 1  |  Page : 138-145

Gas chromatography–mass spectrometry for quantitative and qualitative analysis of essential oil from Curcuma wenyujin rhizomes


1 Key Laboratory of Resources Conservation and Development of Southern Medicine of Hainan Province & Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Haikou 570311, China
2 School of Life and Pharmaceutical Sciences, Hainan University, Haikou 570228, China
3 Key Laboratory of Resources Conservation and Development of Southern Medicine of Hainan Province & Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Haikou 570311; Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China

Correspondence Address:
Prof. Mei-Hua Yang
Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 4,MYaogu 4 Road, Xiuying District, Haikou, Hainan Province 570311
China
Prof. Xiang-Sheng Zhao
Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 4, Yaogu 4 Road, Xiuying District, Haikou, Hainan Province 570311
China
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/wjtcm.wjtcm_87_20

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Objectives: A rapid and sensitive gas chromatography–mass spectrometry (GC–MS) method for quantitative and qualitative analysis of essential oil from Curcuma wenyujin rhizomes was established. Methods: The essential oil of C. wenyujin rhizomes was extracted by supercritical CO2 extraction (SFE). Six main bioactive compounds (eucalyptol, β-elemene, curzerene, germacrone, curdione, and curcumol) were analyzed in selected ion monitoring mode (SIM). Results: Curzerene is not originally present in C. wenyujin rhizomes, but is a product of the transformation of furanodiene at high temperature. The six target components demonstrated good linearity (R2 > 0.9979) over a relatively wide concentration range. The interday and intraday variations had relative standard deviation values less than 5% and the average recovery ranged from 96.95% to 100.04%. The limit of quantitation ranged from 0.032 to 0.235 μg/mL. The developed method was successfully used to analyze the six compounds in 17 samples collected from different origins. Significant variation was observed for the concentrations of the six compounds. In addition, 51 constituents were identified in C. wenyujin rhizome essential oil, consisting of 87.66% of the total essential oil, including curdione, curzerene, dehydrocurdione, germacrone, 1,4-bis(2-benzimidazoyl)benzene, neocurdione, curcumenone, and β-elemene. Conclusions: The proposed method will be useful in the quality control of C. wenyujin rhizome essential oil production.


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