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ORIGINAL ARTICLE
Year : 2021  |  Volume : 7  |  Issue : 1  |  Page : 6-10

Simultaneous quantitative analysis of five components in Angelica sinensis and Angelica acutiloba acclimatized growing in vietnam by high-performance liquid chromatography with photodiode array detector


1 Department of Microbiology, Institute of Drug Quality Control, Ho Chi Minh City, Vietnam
2 Department of Microbiology; Department of Instrumental Analysis, Institute of Drug Quality Control, Ho Chi Minh City, Vietnam
3 Department of Instrumental Analysis, Institute of Drug Quality Control; Institute of Drug Quality Control, Ho Chi Minh City, Vietnam
4 Department of Microbiology, Institute of Drug Quality Control; Faculty of Pharmacy, Nguyen Tat Thanh University, Ho Chi Minh City, Vietnam

Correspondence Address:
Dr. Thi Minh Tam Phama
Institute of Drug Quality Control, 200 Co Bac Street, District 1, Ho Chi Minh City
Vietnam
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/wjtcm.wjtcm_28_20

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Background: Chinese Danggui (Angelica sinensis) and Japanese Danggui (Angelica acutiloba) are evaluated as the same in using and critical of quality control in Vietnamese Pharmacopoeia. In Vietnam, Japanese Danggui were acclimatized and cultivated in several regions in recent years. Despite the huge climatic difference between Vietnam and Japan, Japanese Danggui grows very well in Vietnam. However, there are no studies to assess the overall quality of this medicinal herbs. Aims and Objectives: The aim of this study is to develop a method to identify and assay simultaneously 5 component compounds of these crude herbs: chlorogenic acid, ferulic acid, scopoletin, xanthotoxin and ligustilide by high-performance liquid chromatography with photodiode array detector (HPLC-PDA). Materials and Methods: The procedure was optimized by using column Phenomenex Gemini 5 μm PR C18, 15 x 4,6 mm with the detection wavelength set at 321 nm for detector PDA and mobile phase was composed of (A) ACN and (B) aqueous solution 1% of acid acetic using a gradient elution. Analytes were performed at 30°C with a flow rate of 1.0 mL/min. Results: The developped method were fully validated for linearity, accuracy, recovery and met the validation requirements, proved practical applicability for the quality control of these herbs and related products. Conclusion: The method was successfully applied to the quantification of five markers simultaneously from collected samples.


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