• Users Online: 277
  • Print this page
  • Email this page
ORIGINAL ARTICLE
Year : 2021  |  Volume : 7  |  Issue : 1  |  Page : 78-85

Identification and determination of fructooligosaccharides in snow chrysanthemum ( Coreopsis tinctoria nutt.)


State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao SAR, China

Correspondence Address:
Prof. Jing Zhao
State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao SAR
China
Prof. Shao-Ping Li
State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao SAR
China
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/wjtcm.wjtcm_64_20

Rights and Permissions

Objective: Small molecules in snow chrysanthemum such as flavonoids, phenolic compounds and amino acids have been extensively investigated. No study to date has focused on water-soluble oligosaccharides. The objective of this study is identification and determination of water-soluble oligosaccharides in snow chrysanthemum. Methods: The oligosaccharides in snow chrysanthemum were identified by high performance thin layer chromatography (HPTLC), liquid chromatography-mass spectrometry (LC-MS) combined MS library and methylation analysis for the first time. Subsequently the oligosaccharides were determined by high performance liquid chromatography with a charged aerosol detector (HPLC-CAD). Results: The oligosaccharides in snow chrysanthemum were identified as inulin-type fructooligosaccharides (FOS). The yield of FOS (DP3~DP13) in the first extraction was over 97.6%. The RSDs of repeatability in three sample amount levels (0.08 g, 0.1 g, 0.12 g) are lower than 4.8% and the RSDs of stability are less than 3.5%. The recoveries of FOS (DP3~13) were ranging from 96.9% to 105.6%. The contents of FOS (DP3~DP13) in flowers of snow chrysanthemum from different regions of China were greatly variant. Conclusion: This is the first time to identify and quantify FOS in snow chrysanthemum which is helpful for its performance in the in the fields of biomedical, agriculture and functional food industry as well as development of the quality control methods. In addition, the identification approach developed in this work can also be used for screening potential natural sources containing FOS..


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed186    
    Printed0    
    Emailed0    
    PDF Downloaded23    
    Comments [Add]    

Recommend this journal