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ORIGINAL ARTICLE
Year : 2021  |  Volume : 7  |  Issue : 2  |  Page : 246-253

Pharmacokinetic and relative bioavailability of three secoiridoid glycosides in beagle dog plasma after oral administration of conventional and enteric-coated capsules of Gentianella acuta extract


1 College of Pharmacy, Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin 150040, China
2 SunGen Pharma, LLC, 303C Collage Road East, Princeton, NJ08540, USA

Correspondence Address:
Prof. Hai-Xue Kuang
Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, 24 Heping Road, Xiangfang District, Harbin 150040
China
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/wjtcm.wjtcm_10_21

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Objective: To simultaneously investigate the pharmacokinetics of gentiopicroside, sweroside, and swertiamarin, which are constituents of Gentianella acuta, by developing and validating a simple, sensitive, and fast ultra-high-performance liquid chromatography–tandem mass spectrometry method. Materials and Methods: Blood samples were collected from the forward limb veins of six beagle dogs following oral gavage with G. acuta, the whole plant extract (39.90 mg/kg). Plasma samples were processed using liquid–liquid extraction. The analytes and paeoniflorin (internal standard [IS]) were separated using an Acquity® UPLC ethylene bridged hybrid amide column (2.1 mm × 100 mm, 1.7 μm) with isocratic elution using a mobile phase consisting of acetonitrile and 0.1% formic acid in water (80:20, v/v) at a flow rate of 0.4 mL/min. Quantification was performed using multiple reaction monitoring of the fragmentation transitions at m/z 401.1s594;179.0, 403.1→195.0, 419.1→179.0, and 525.2→449.1 for gentiopicroside, sweroside, swertiamarin, and the IS, respectively. Results: The linearity of the analytical response was good and the calibration curves were linear over concentration ranges of 1.20–192.0, 0.40–159.0, and 0.20–209.3 ng/mL for gentiopicroside, sweroside, and swertiamarin, respectively. The extraction recovery was in the range of 84.72%–91.34%, 84.58%–93.43%, and 82.75%–91.37% for gentiopicroside, sweroside, and swertiamarin, respectively. Conclusions: The method was successfully used to evaluate the pharmacokinetic parameters of gentiopicroside, sweroside, and swertiamarin in beagle dogs.


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