World Journal of Traditional Chinese Medicine

ORIGINAL ARTICLE
Year
: 2021  |  Volume : 7  |  Issue : 2  |  Page : 246--253

Pharmacokinetic and relative bioavailability of three secoiridoid glycosides in beagle dog plasma after oral administration of conventional and enteric-coated capsules of Gentianella acuta extract


Zhi-Bin Wang1, Kai Li1, Meng Wang1, Gao-Song Wu1, Yu-Jin Bi2, Hai-Xue Kuang1 
1 College of Pharmacy, Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin 150040, China
2 SunGen Pharma, LLC, 303C Collage Road East, Princeton, NJ08540, USA

Correspondence Address:
Prof. Hai-Xue Kuang
Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, 24 Heping Road, Xiangfang District, Harbin 150040
China

Objective: To simultaneously investigate the pharmacokinetics of gentiopicroside, sweroside, and swertiamarin, which are constituents of Gentianella acuta, by developing and validating a simple, sensitive, and fast ultra-high-performance liquid chromatography–tandem mass spectrometry method. Materials and Methods: Blood samples were collected from the forward limb veins of six beagle dogs following oral gavage with G. acuta, the whole plant extract (39.90 mg/kg). Plasma samples were processed using liquid–liquid extraction. The analytes and paeoniflorin (internal standard [IS]) were separated using an Acquity® UPLC ethylene bridged hybrid amide column (2.1 mm × 100 mm, 1.7 μm) with isocratic elution using a mobile phase consisting of acetonitrile and 0.1% formic acid in water (80:20, v/v) at a flow rate of 0.4 mL/min. Quantification was performed using multiple reaction monitoring of the fragmentation transitions at m/z 401.1s594;179.0, 403.1→195.0, 419.1→179.0, and 525.2→449.1 for gentiopicroside, sweroside, swertiamarin, and the IS, respectively. Results: The linearity of the analytical response was good and the calibration curves were linear over concentration ranges of 1.20–192.0, 0.40–159.0, and 0.20–209.3 ng/mL for gentiopicroside, sweroside, and swertiamarin, respectively. The extraction recovery was in the range of 84.72%–91.34%, 84.58%–93.43%, and 82.75%–91.37% for gentiopicroside, sweroside, and swertiamarin, respectively. Conclusions: The method was successfully used to evaluate the pharmacokinetic parameters of gentiopicroside, sweroside, and swertiamarin in beagle dogs.


How to cite this article:
Wang ZB, Li K, Wang M, Wu GS, Bi YJ, Kuang HX. Pharmacokinetic and relative bioavailability of three secoiridoid glycosides in beagle dog plasma after oral administration of conventional and enteric-coated capsules of Gentianella acuta extract.World J Tradit Chin Med 2021;7:246-253


How to cite this URL:
Wang ZB, Li K, Wang M, Wu GS, Bi YJ, Kuang HX. Pharmacokinetic and relative bioavailability of three secoiridoid glycosides in beagle dog plasma after oral administration of conventional and enteric-coated capsules of Gentianella acuta extract. World J Tradit Chin Med [serial online] 2021 [cited 2022 Jul 7 ];7:246-253
Available from: https://www.wjtcm.net/article.asp?issn=2311-8571;year=2021;volume=7;issue=2;spage=246;epage=253;aulast=Wang;type=0